毛细管电泳电化学法检测过氧化氢  

DETERMINATION OF HYDROGEN PEROXIDE BY CAPILLARY ELECTROPHORESIS WITH AMPEROMETRIC DETECTION

作　　者：董冉冉[1] 张燕[1] 时克林 马艳芳[1] 李玲君[1] 王晓蕾[1] 

机构地区：[1]山东师范大学化学化工与材料科学学院,济南250014 [2]淄博六中,淄博252300

出　　处：《化学分析计量》2011年第6期26-29,共4页Chemical Analysis And Meterage

摘　　要：以金微盘电极和离子液体修饰单壁碳纳米管糊微盘电极分别作为毛细管电泳电化学检测器,试验了两种电极对过氧化氢的响应情况,将金微盘电极与毛细管电泳联用,对过氧化氢进行了定性和定量检测。探讨了分离电压、缓冲溶液pH值和工作电位等条件对H2O2检测的影响。实验结果表明,峰电流与H2O2浓度在1.0×10–6～1.0×10–5mol/L和3.0×10–5～1.0×10–3mol/L呈良好的线性,线性回归方程分别为y(nA)=0.041 78+0.015 5x(μmol/L),y(A)=–3.304 5×10–4+0.024 29x(mmol/L),相关系数分别为0.997 5和0.999 0,当信噪比S/N=3时,浓度检出限为5×10–7mol/L。实验中发现,样品中的多巴胺、抗坏血酸等对H2O2的测定无干扰。Gold microelectrode and ionic liquid modified-SWNTs paste microelectrode made by ourselves were used in capillary zone electrophoresis with electrochemical end-column amperometric detection to determine hydrogen peroxide. Gold microelectrode showed higher sensitivity to H2O2 was used as the detector of capillary electrophoresis. Factors influencing the performance, including separation voltage, detection potential and pH value of the buffer were studied. Under optimized detection conditions, hydrogen peroxide responded linearly from 1.0× 10-5-1 × 10-3 mol/L and 3.0 × 10-5-1.0 × 10-3 with a correlation coefficient of 0.997 5 and 0.999 0 respectively. The concentration limit of detection of the method was 5 × 10-7 mol/L （ S/N=3 ）. The experiment also showed that there was no electroactive interferences such as dopamine and ascorbie acid.

关 键 词：金微盘电极 碳纳米管糊微盘电极 毛细管电泳 电化学检测 过氧化氢 

分 类 号：O657.1[理学—分析化学]