茉莉酸甲酯对干旱及复水条件下烤烟幼苗叶绿素荧光特性的影响  

Effects of foliar spraying methyl jasmonate on leaf chlorophyll fluorescence characteristics of flue-cured tobacco seedlings under drought and re-watering.

作　　者：金微微[1] 王炎[1] 张会慧[1] 焦志丽[1] 王鹏[1] 李鑫[1] 岳冰冰[1] 孙广玉[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《应用生态学报》2011年第12期3157-3162,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(30771746;31070307);黑龙江省烟草专卖局项目(2007;2009)资助

摘　　要：以烤烟品种"龙江911"为试验材料,研究了干旱及复水过程中外源茉莉酸甲酯(Me-JA)对移栽后烤烟幼苗叶绿素含量和叶绿素荧光特性的影响.结果表明:干旱下烤烟幼苗叶绿素含量、PSⅡ反应中心完全关闭时荧光产量(Fm)、PSⅡ潜在活性(Fv/Fo)、最大光化学效率(Fv/Fm)、实际光化学效率(ФPSⅡ)、表观电子传递速率(ETR)和光化学猝灭系数(qP)下降,而初始荧光(Fo)和非光化学猝灭系数(qN)升高,0.2和0.5mmol.L-1的外源MeJA明显减缓了干旱下烤烟幼苗Fv/Fm、Fv/Fo、ФPSⅡ、ETR、qP的下降和qN的上升,而1.0mmol.L-1MeJA效果不明显.复水后,烤烟幼苗各项叶绿素荧光指标均有明显恢复,并且MeJA处理后的幼苗恢复更明显.表明外源MeJA减轻了干旱胁迫下烤烟叶片叶绿素的分解,对PSII反应中心起到一定的保护作用,提高了电子传递速率,降低了干旱胁迫对烤烟幼苗的伤害,并且复水后叶绿素含量和叶绿素荧光参数能迅速恢复,从而保证了经干旱胁迫后烤烟幼苗能迅速缓苗.Taking the flue-cured tobacco variety of ＂Longjiang 911＂ from Heilongjiang Province of Northeast China as test material,a pot experiment was conducted to study the effects of foliar spraying different concentration methyl jasmonate（MeJA） on the seedlings leaf chlorophyll content and chlorophyll fluorescence characteristics during the transplantation stage under drought and re-watering.Under drought condition,the leaf chlorophyll content,maximum fluorescence（Fm）,potential activities of PSII（Fv/Fo）,maximum photochemical efficiency（Fv/Fm）,actual photochemical efficiency（ФPSⅡ）,apparent electron transport rate（ETR）,and photochemical quenching（qP） decreased,but the minimal fluorescence（Fo） and non-photochemical quenching（qN） increased.Foliar spraying 0.2 and 0.5 mmol·L-1 of MeJA had obvious positive effects in mitigating the decrease of Fv/Fm,Fv/Fo,ФPSⅡ,ETR,and qP and the increase of qN under drought stress,while spraying 1.0 mmol·L-1 of MeJA had lesser effects.After re-watering,all the leaf chlorophyll fluorescence indices had obvious recovery,and spraying MeJA made the indices more close to the original levels before drought.It was suggested that foliar spraying MeJA could alleviate the degradation of chlorophyll and play definite role in protecting the PSII under drought stress,decrease the damage of drought stress on the seedlings,promote the rapid MeJA recovery of chlorophyll fluorescence parameters after re-watering,and thus,ensure the regrowth of flue-cured tobacco seedlings.

关 键 词：烤烟 干旱和复水 茉莉酸甲酯 叶绿素荧光特性 

分 类 号：S572[农业科学—烟草工业]