肺虚痰阻证模型大鼠肺组织αENaC分子表达与补肺化痰方对其影响  

Expression of αENaC in lungs of rats with "phlegm obstruction due to lung-deficiency syndrome" and effects of Bufei Huatan Decoction

作　　者：江湧[1] 李小兵[1] 刘小虹[1] 王丽新[1] 任明能[1] 吴启端[1] 谢宇晖[1] 吴建奇[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405

出　　处：《中成药》2011年第11期1864-1868,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(30772687);广东省自然科学基金面上项目(9251040701000001)

摘　　要：目的通过观察肺虚痰阻证模型αENaC的基因、蛋白表达,及补肺化痰中药复方(人参、制南星、制半夏、枳实、橘红、茯苓、石菖蒲、竹茹和炙甘草)治疗前、后的对比,动态观察这一过程中上述指标的变化情况。方法将60只雄性SD大鼠随机分为正常组、模型组、中药治疗组。模型组和治疗组采用烟熏方法造模40 d,治疗组在造模26 d后,药物灌胃治疗2周。采用免疫组化染色法,检测大鼠肺组织αENaC的分布及变化;RT-PCR的方法检测大鼠肺组织中αENaC基因表达;western blot法检测大鼠肺组织中αENaC蛋白水平。结果免疫组化结果显示,模型组肺组织中αENaC的阳性率显著高于正常组(P<0.001)和治疗组(P<0.05),治疗组和正常组差异显著,治疗组高于正常组(P<0.05);mRNA、蛋白表达结果显示,模型组α-ENaC基因、蛋白表达量高于正常组(P<0.01);治疗组亦高于正常组(P<0.05)但较模型组低(P<0.05)。结论肺虚痰阻证模型大鼠肺泡上皮组织α-ENaC基因及蛋白表达量升高,可能是肺虚痰阻证的病理机制之一;补肺化痰中药复方可减少肺虚痰阻证模型大鼠肺组织α-ENaC基因及蛋白表达。AIM To use the basic molecular parameters of genomic expression and protein expression of αENaC to study the rat model for ＂phlegm obstruction due to lung-deficiency syndromes＂ and to observe the dynamic changes of the parameters after medication with Bufei Huatan Decoction.METHODS Sixty male SD rats were randomized into three groups： normal,model,and treatment group（with herbal compound）.Model and treatment groups were induced continuously by fumigation for 40 days.After 26 days of induction,the treatment group was given Bufei Huatan Decoction by intragastric administration for two weeks.The mRNA and protein expressions of αENaC in lung were assayed by semi-quantitative RT-PCR and Western blot as well as immunohistochemical staining,respectively.RESULTS Immunohistochemical staining assay showed that the positive rate of αENaC protein expression in lung tissues of the model group was significantly higher than those of normal and treatment groups（P〈0.001）.In the treatment group,protein expression was remarkably increased over the normal group（P〈0.05）;mRNA and protein expression of αENaC were significantly higher in the model group compared with the normal group（P〈0.01）.The same parameters were significantly higher in the treatment group when compared with the normal group（P〈0.05）,but lower than the model group（P〈0.05）.CONCLUSION These results suggest that one of the pathological mechanisms of the syndrome of ＂phelgm obstuction due to lung deficiency＂ may be caused by the upregulation ofαENaC gene and protein expression lungs of the alveolar epithelium of rats;Bufei Huatan Decoction downregulates the gene and protein expression of αENaC in the lungs of model rats.

关 键 词：αENaC 大鼠 肺 肺虚痰阻证 补肺化痰中药复方 

分 类 号：R285.5[医药卫生—中药学]