HPLC法同时测定透骨灵橡胶膏中槲皮素、原儿茶酸和乌头碱  

Simultaneous determination of quercetin,protocatechuic acid and aconitine in Touguling Adhesive Plaste by HPLC

作　　者：贾晓斌[1,2] 朱静[1,2] 黄洋[1,3] 郑智音[1,3] 

机构地区：[1]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028 [2]南京中医药大学,江苏南京210046 [3]江苏大学药学院,江苏镇江212013

出　　处：《中成药》2011年第11期1927-1930,共4页Chinese Traditional Patent Medicine

基　　金：国家"十一五"科技支撑计划项目(2008BAI53B072)

摘　　要：目的建立同时测定透骨灵橡胶膏(透骨草、天南星、鸡血藤等)中槲皮素,原儿茶酸和乌头碱的测定方法。方法采用反向高效液相色谱法,色谱柱为Agilent C18色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.2%磷酸(梯度洗脱),检测波长为250 nm,体积流量为1 mL/min。结果槲皮素在0.158 8～2.540 8μg,原儿茶酸在0.177 6～2.841 6μg/mL,乌头碱在0.212 8～3.404 8μg/mL与峰面积呈良好的线性关系。加样回收率分别为98.6%、100.8%、97.7%,RSD分别为1.66%、2.58%、1.09%。结论能准确分析透骨灵橡胶膏中3种主要成分,灵敏度高,重复性好,可用来控制透骨灵橡胶膏的质量。AIM To establish a method for simultaneously determining quercetin,protocatechuic acid and aconi-tine in Touguling Adhesive Plaste（Speranskiae seu Impatientis Herba,Arisaematis Rhizoma,Spatholobi Caulis,etc.） by HPLC.METHODS RP-HPLC was carried out on the chromatographic column（150 mm × 4.6 mm,5 μm） with acetonitrile-0.2% phosphoric acid as mobile phase（gradient elution）.The UV detection was set at 250 nm and the flow rate was 1.0 mL/min.RESULTS The calibration curve of quercetin was linear in the range of 2.01-52.76 μg/mL,protocatechuic acid was in the range of 5.08-125.39 μg/mL and aconitine was in the range of 5.32-125.77 μg/mL.The average recoveries were 98.6%,100.8% and 97.7%,respectively,with the RSDs of 1.66%,2.58% and 1.09%.CONCLUSION The method can analyse the three components accurately.It is accurate,reproducible and can be used to control the contents of Touguling Adhesive Plaste.

关 键 词：透骨灵橡胶膏 RP-HPLC 槲皮素 原儿茶酸 乌头碱 

分 类 号：R927.2[医药卫生—药学]