VIPR-1基因SNP位点与如皋黄鸡产蛋性能和蛋品质的关联分析  

Association of SNP of VIPR-1 Gene with Egg Production and Egg Quality Traits in Rugao-yellow Chicken

作　　者：洪军[1] 王克华[2] 李东锋[3] 杨宁[3] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省家禽科学研究所,江苏扬州225125 [3]中国农业大学动物科技学院,北京100193

出　　处：《中国畜牧杂志》2011年第23期1-5,共5页Chinese Journal of Animal Science

基　　金：现代农业产业技术体系建设专项资金(CARS-41);江苏省科技支撑项目(BE2010374);江苏省农业科技自主创新资金项目(cx(10)122);江苏省"333工程"培养资金项目;国家农业科技成果转化资金项目(2010C10020605)

摘　　要：本研究旨在分析VIPR-1基因的SNP位点与产蛋性能和蛋品质之间的关联性。实验以如皋黄鸡为素材,采用飞行时间质谱技术对VIPR-1基因内含子2第+1063位碱基(PA位点)的G-T突变和第+3522位碱基(PB位点)的T-C突变进行单位点和多位点组合效应分析。结果表明:PA位点的多态性与产蛋性状中的开产日龄(AFE)和40周龄总产蛋数(EN40)呈显著相关(P<0.05);与蛋品质性状中的蛋壳强度(ESS)、蛋黄比率(YR)和蛋黄蛋白比(YAR)呈显著相关(P<0.05);等位基因T有利于产蛋量和蛋品质的改善。PB位点与本次实验所测的5个产蛋性状相关均不显著(P>0.05),与蛋品质性状中的蛋重(EW)、蛋形指数(ESI)、蛋壳厚度(EST)和蛋比重(ESG)相关显著(P<0.05)。组合效应分析结果表明,对于产蛋性能,GTTT和TTTC组合基因型个体的组合效应显著。This study was conducted to analyze the effect of genotype combinations between the single nucleotide polymorphisms（SNPs） of vasoactive intestinal peptide receptor-1（VIPR-1） gene on the egg production and egg quality traits in Rugao-yellow Chicken.410 purebred Rugao-yellow Chicken were used.The SNPs of the base mutation G-T at position 1063（PA site） and mutation C-T at position 3522（PBsite）in the intron of PRL gene were detected by SELDI-TOF.Association analyses showed that the PA site was significantly associated with the age of first egg（AFE）,the total number of egg of 40 w（EN40）,egg shell strength（ESS）,yolk rate（YR）and yolk-albumen rate（YAR）（P0.05）.Allele T was positive for number of egg and egg quality traits（P0.05）.The PB site was not significantly associated with egg production traits（P0.05）,was significantly associated with egg weight（EW）,egg shape index（ESI）,egg specific gravity（ESG）,egg shell thickness（EST）of the egg quality traits（P0.05）.The result of genotype combinations effect suggested that among nine different genotype combinations,GTTT and TTTC genotypes were the dominant combination with the egg production traits.

关 键 词：VIPR-1 产蛋性能 蛋品质 如皋黄鸡 

分 类 号：S831.2[农业科学—畜牧学]