丹芍化纤胶囊对肺纤维化干预作用及机制  

Effects of Danshaohuaxian capsule on pulmonary fibrosis and its mechanism in rats

作　　者：谢汝佳[1] 韩冰[1] 张成俊[1] 何小飞[1] 杨婷[1] 杨勤[1] 

机构地区：[1]贵阳医学院病理生理学教研室,贵州贵阳550004

出　　处：《中国公共卫生》2011年第12期1539-1541,共3页Chinese Journal of Public Health

基　　金：贵州省科技攻关项目(黔科合SZ字[2008]3049号);贵州省科技厅国际科技合作项目(黔科合外G字[2010]7016号)

摘　　要：目的探讨calpain-2与caspase-3在肺纤维化中的变化及丹芍化纤胶囊对肺纤维化的干预作用机制。方法选取SD大鼠30只,随机分为对照组、模型组和丹芍组,每组各10只,模型组和丹芍组气管内一次性灌注博来霉素5 mg/kg诱导肺纤维化,丹芍组于造模第2 d给予丹芍化纤胶囊混悬液灌胃(0.8 g/kg),于实验第28 d处死大鼠;采用免疫组织化学方法检测肺组织中calpain-2与caspase-3蛋白的表达情况;运用荧光实时定量PCR(real-time PCR)检测肺组织中calpain-2及caspase-3 mRNA的表达变化。结果模型组大鼠肺组织中calpain-2,caspase-3的蛋白及mR-NA表达分别为(0.35±0.06)、(0.41±0.12)、(210±86)、(614±106),明显高于对照组(P<0.01);经丹芍化纤胶囊干预后,肺组织中calpain-2蛋白(0.18±0.03)、mRNA(97±71)及caspase-3蛋白(0.22±0.04)、mRNA(315±92)的表达较模型组明显减少(P<0.05或P<0.01)。结论 calpain-2及caspase-3在肺纤维化的发生发展中作用明显,丹芍化纤胶囊干预肺纤维化的机制可能与减少肺组织中calpain-2与caspase-3的表达有关。Objective To investigate the effect of calpain-2 and caspase-3 on pulmonary fibrosis and the protective effect of Danshaohuaxian（DSHX） capsule on pulmonary fibrosis in rats.Methods Thirty SD rats were randomly divided into control group,pulmonary fibrosis model group,and DSHX capsule treatment group.Pulmonary fibrosis model was induced by intratracheal instillation of single dose bleomycin（5 mg/kg）.DSHX capsule of 0.8 g/kg was administered daily by oral to the treatment group at the second day after bleomycin exposure.All rats were killed on the 28th day.Immunohistochemistry was used to detect the expression of calpain-2 and caspase-3 protein.The expression of calpain-2 and caspase-3 mRNA were detected by real-time PCR.Results The expression of calpain-2 protein（0.35±0.06）,calpain-2 mRNA（210±86）,caspase-3 protein（0.41±0.12）,and caspase-3 mRNA（614±106）in the rats of molel group were higher than those in the control group（P0.01）.After the treatment of DSHX capsule,the expression of calpain-2 protein（0.18±0.03）,calpain-2 mRNA（97±71）,caspase-3 protein（0.22±0.04）,and caspase-3 mRNA（315±92） were lower than those in the model group（P0.05,P0.01）.Conclusion Calpain-2 and caspase-3 play an important role in the process of pulmonary fibrosis.DSHX capsule could reduce pulmonary fibrosis induced by bleomycin,and its mechanisms may include the expression of calpain-2 and caspase-3.

关 键 词：肺纤维化 丹芍化纤胶囊 钙激活中性蛋白酶2 CASPASE-3 

分 类 号：R563[医药卫生—呼吸系统]