林蛙油冲剂对微波辐射大鼠学习记忆影响  

Effect of Rana japonica oil compound granules on learning/memory ability of rats exposed to microwave radiation under hypergravity environment

作　　者：陈默然[1] 高俊涛[1] 李妍[1] 李强[1] 赵行宇[1] 任旷[1] 沈楠[1] 潘文干[1] 

机构地区：[1]吉林医药学院生理学教研室,吉林吉林132013

出　　处：《中国公共卫生》2011年第12期1591-1593,共3页Chinese Journal of Public Health

基　　金：吉林省科技厅白求恩基金(200705408)

摘　　要：目的观察林蛙油复方冲剂对超重环境下微波辐射大鼠学习记忆能力影响。方法制备Wistar大鼠超重环境下微波辐射模型,采用林蛙油冲剂进行干预,连续14 d,Morris水迷宫实验观察学习记忆能力改变,western blot检测大脑皮层热休克蛋白70(HSP70)表达。结果与空白对照比较,模型组定位航行潜伏期由(12.03±1.85)s延长至(32.54±5.75)s(P<0.05),跨平台次数由(6.45±1.35)次/min减少至(2.16±1.02)次/min(P<0.05),脑组织HSP70表达明显升高(P<0.05);与模型组比较,林蛙油冲剂辐射前处理组定位航行潜伏期缩短至(13.88±5.93)s(P<0.05),跨平台次数增加至(5.91±1.53)次/min(P<0.05),脑组织HSP70蛋白表达降低(P<0.05)。结论林蛙油复方冲剂可改善超重环境下微波辐射模型大鼠学习记忆能力,其机制可能与降低大脑皮层组织HSP70表达有关。Objective To assess the effect of Rana japonica oil compound granules on the ability of learning/memory of the rats exposed to microwave radiation under hypergravity environment.Methods The model of Wistar rats under synergistic effect of hypergravity environment and microwave radiation was established and the rats were intervened with Rana japonica oil compound granules for 14 days.Morris water maze was used to detect the ability of learning and memory of the rats and the expression of heat shock protein 70（HSP70） in the cortex of the rats were determined with western blot.Results Compared with blank control group,the escape latency of the rats in model group was significantly prolonged from 12.03±1.85 seconds to 32.54±5.75 seconds（P0.05） and the number of finding the platform was decreased remarkably from 6.45±1.35 per minute to 2.16±1.02 per minute（P0.05）.The expression of HSP70 in the cortex increased significantly（P0.05） and significantly decreased in compound granules protection group before hypergravity and radiation treatment（P0.05）.Compared with the model group,the escape latency was shortened to 13.88±5.93 seconds（P0.05） and the number of finding the platform increased to 5.91±1.53 per minute（P0.05） in compound granules protection group before hypergravity and radiation treatment.Conclusion Rana japonnica oil compound granules can facilitate spatial learning and memory of the rats exposed to microwave radiation under hypergravity environment through reducing the expression of HSP70 in the cortex.

关 键 词：超重 微波辐射 林蛙油 学习记忆 MORRIS水迷宫 热休克蛋白70 

分 类 号：R285.5[医药卫生—中药学]