乙二胺四乙酸铵梯度法制备盐酸伊立替康脂质体  

Preparation of irinotecan hydrochloride liposomes by ammonium ethylenediaminetetraacetate gradient method

作　　者：李哲[1] 牟丽娜[2] 王绍宁[3] 杨强[1] 邓意辉[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]辽源市中医院,吉林辽源136200 [3]沈阳药科大学制药工程学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2011年第12期933-937,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的制备盐酸伊立替康脂质体,考察包封率及粒径的影响因素。方法采用乙二胺四乙酸铵梯度法制备盐酸伊立替康脂质体,以阳离子交换树脂分离脂质体和游离药物,并以紫外分光光度法和激光粒度仪分别测定盐酸伊立替康脂质体的包封率和粒径;考察不同处方和制备工艺对脂质体包封率及粒径的影响。结果通过处方工艺优化,盐酸伊立替康脂质体包封率达到95.73%,粒径为112.8 nm。结论以乙二胺四乙酸铵梯度法制备的盐酸伊立替康脂质体包封率较高,方法可行。Objective To prepare irinotecan hydrochloride （CPT-11 ）liposomes, and study the influence of factors on the entrapment efficiency and size. Methods CPT-11 was encapsulated in the liposomes by ammonium ethylenediaminetetraacetate（NH4EDTA） gradient method. Free CPT-11 and liposomes were separated by cation exchange resin. Ultraviolet spectrophotometry and laser particle analyzer were applied to determine the entrapment efficiency and the size of CPT-11 liposomes, respectively. The influence of various factors on the entrapment efficiency and size of CPT-11 liposomes were investigated. Results Through optimizing the formulations and preparation conditions, the CPT-11 liposomes were with high entrapment efficiency to 95.73% and the mean diameter to 112. 8 nm. Conclusions The CPT-11 liposomes with high entrapment efficiency can be prepared by NH4EDTA gradient method, and the method is proved to be feasible.

关 键 词：盐酸伊立替康 乙二胺四乙酸铵梯度法 脂质体 包封率 粒径 

分 类 号：R94[医药卫生—药剂学]