HPLC法同时测定芩连片中黄芩苷﹑黄芩素和汉黄芩素的含量  

Simultaneous determination of baicalin,baicalein and wogonin in Qinlian tablets by HPLC

作　　者：王冬梅[1] 高靥[1] 张洪霞[1] 金艺[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2011年第12期955-958,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立同时测定芩连片中黄芩苷、黄芩素和汉黄芩素含量的HPLC方法。方法采用Dia-monsil C18柱(200 mm×4.6 mm,5μm)分离,以甲醇-体积分数为0.4%的磷酸溶液为流动相梯度洗脱,流速为1.0 mL.min-1,检测波长为277 nm,柱温为35℃。结果黄芩苷、黄芩素及汉黄芩素的线性范围分别为36.8～368.0 mg.L-1(r=0.999 7)、1.75～17.5 mg.L-1(r=0.999 8)、1.50～15.0mg.L-1(r=0.999 9),黄芩苷、黄芩素、汉黄芩素的平均回收率分别为97.3%(RSD=1.8%)、101.1%(RSD=1.8%)、99.0%(RSD=2.0%)。结论 HPLC法可用于芩连片的质量控制。Objective To establish an RP-HPLC method for determination of baicalin, baicalein and wogonin in Qinlian （traditional Chinese medicines）tablets. Methods The samples were separated on a Diamonsil ODS colunm（200 mm ×4.6mm,5μm）through gradient delivery of a mixture of methanol-0. 4% （φ）phosphoric acid solution, the flow rate was 1.0 mL. min-1. The detection wave-length was set at 277 nm and the temperature of column was 35 ℃. Results The linear ranges of baicalin, baicalein and wogonin were 36. 8- 368.0 mg.L-1（r=0. 999 7） ,1.75-17.5 mg.L-1（r=0. 999 8） ,and 1.50-15.0 mg.L-1（r =0. 999 9） ,respectively. The average recoverys of the three components were 97.3% （RSD = 1.8% ）, 101.1% （RSD = 1.8% ） ,99. 0% （RSD = 2. 0% ）, respectively. Conclusions The method is accurate and reproducible. It can be applied for the quality control of Qinlian tablets.

关 键 词：高效液相色谱法 芩连片 黄芩苷 黄芩素 汉黄芩素 

分 类 号：R917[医药卫生—药物分析学]