单生卵囊藻(Oocystis solitaria)和月牙藻(Selenastrum sp.)的培养条件及其细胞组成  

Culture condition and cellular biochemical composition of two microalgae Oocystis solitaria and Selenastrum sp.

作　　者：严佳琦[1] 黄旭雄[1] 马珅俊[1] 黄征征[1] 吕为群[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海200090

出　　处：《生态学杂志》2011年第12期2761-2766,共6页Chinese Journal of Ecology

基　　金：上海市农委重点攻关项目(沪农科攻字2005第4-4号);国家海洋局课题(SHME2011SW02);国家高技术研究发展计划项目(2009AA064401);上海市重点学科建设项目(Y1101)资助

摘　　要：从海滨的淡水池沼中分离得到单生卵囊藻(Oocystis solitaria)和月牙藻(Selenastrum sp.),分别研究了温度、光照强度、盐度对2种微藻生长繁殖的影响,分析了2种微藻的细胞组成、脂肪酸组成及盐度对2种微藻脂肪积累的影响。结果表明:单生卵囊藻和月牙藻的适宜生长温度分别为35.9℃～40.5℃和29.7℃～32.8℃;单生卵囊藻和月牙藻的适宜光照强度分别为46～70&mol·m-2·s-1和17～54&mol·m-2·s-1;单生卵囊藻在淡水培养液中生长最好,月牙藻在盐度为2的半咸水培养液中生长最好;在适宜培养条件下,单生卵囊藻细胞蛋白、总糖和总脂肪分别为27.61%、22.00%和3.84%;月牙藻细胞的蛋白、总糖和总脂肪分别为28.06%、21.99%和12.53%;单生卵囊藻的脂肪酸组成中含有丰富的18:3n3;月牙藻的脂肪酸组成中含有DHA;盐度影响2种微藻的总脂肪含量;单生卵囊藻和月牙藻分别在盐度4和10的半咸水培养液中细胞总脂含量最高。Microalgae are the potential resources for producing biological and renewable energy.In this study,two microalgae Oocystis solitaria and Selenastrum sp.were collected from a coastal freshwater pond,and a culture experiment was conducted to determine the optimum water temperature,light intensity,and water salinity for the growth and reproduction of the two microalgae.The cellular biochemical composition of the two microalgae was analyzed,and the effects of water salinity on the lipid content of these microalgae were investigated.The suitable water temperature and light intensity for the growth of O.solitaria and Selenastrum sp.were 35.9-40.5 ℃ and 29.7-32.8 ℃,and 46-70 μmol·m-2·s-1 and 17-54 μmol·m-2·s-1,respectively.O.solitaria grew best in fresh water,while Selenastrum sp.flourished in brackish water at salinity 2.Under suitable culture conditions,the cell protein,carbohydrate,and total lipid contents of O.solitaria were 27.61%,22.00%,and 3.84%,respectively,and those of Selenastrum sp.were 28.06%,21.99% and 12.53%,respectively.In the fatty acid of O.solitaria,18：3n3 was abundant;while in that of Selenastrum sp.,DHA presented.Water salinity affected the total lipid content of the two microalgae.At salinity 4 and 10,O.solitaria and Selenastrum sp.had the highest lipid content,being 8.29% and 18.21%,respectively.

关 键 词：单生卵囊藻 月牙藻 温度 光照强度 盐度 

分 类 号：Q94-3[生物学—植物学]