球形红细菌生物转化槲寄生中总三萜类化合物的测定  

Determination of the total triterpenes in mistletoe transformed by rhodobacter sphaeroides

作　　者：侯晓峰[1] 郑庆红[1] 漆小梅[1] 杨官娥[1] 张肇铭[2] 

机构地区：[1]山西医科大学药学院,山西太原030001 [2]山西大学生命科学与技术学院,山西太原030006

出　　处：《中国医药导报》2011年第35期29-31,共3页China Medical Herald

基　　金：国家自然科学基金(项目编号:30672621);山西省自然科学基金(项目编号:2006011099);山西省自然科学基金(项目编号:2010011048-2);山西医科大学博士启动基金

摘　　要：目的:建立槲寄生及球形红细菌转化槲寄生培养液中总三萜类化合物的含量测定方法,并对各个样品的含量进行比较研究。方法:采用紫外分光光度法,以齐墩果酸为对照品,5%的香草醛-冰醋酸溶液和高氯酸为显色系统,535 nm波长处测定总三萜类化合物的含量。结果:齐墩果酸在2～12μg/ml的范围内线性关系良好,标准曲线回归方程为A=21.988C-0.072,r=0.989 5。75%的乙醇提取、半量常规培养基、游离球形红细菌培养,所得培养液(样品3)总三萜含量和槲寄生75%的乙醇提取液(样品1)相比增加141%。结论:经过球形红细菌转化可以增加槲寄生提取液中总三萜类化合物的含量。Objective： To establish methods for the determination of total triterpenes in mistletoe and its transformation products by rhodobacter sphaeroides,and to compare the contents of each sample.Methods： Oleanolic acid was taken as the standard,the content of total triterpenes was measured by spectrophotometry with 5% vanillin-glacial acetic acid solution and perchloric acid as color-developing agent and the detection wavelength was at 535 nm.Results： The linear correlation of oleanolic acid calibration curve was good in the range of 2-12 μg/ml,and the calibration curve regression equation was A=21.988C-0.072,r=0.989 5.Compared with 75% ethanol extract of mistletoe（sample 1）,the content of total triterpenes in 75% ethanol mistletoe extract cultured with half amount of conventional medium and rhodobacter sphaeroides（sample 3） increased by 141%.Conclusion： The content of total triterpenes in mistletoe extract can be enhanced after biotransformed by rhodobacter sphaeroides.

关 键 词：槲寄生 光合细菌 球形红细菌 三萜类化合物 含量测定 

分 类 号：R284.1[医药卫生—中药学]