HPLC测定复方吴茱萸软膏中吴茱萸碱和吴茱萸次碱的含量  

Simultaneous determination of evodiamine and rutaecarpine of compound Wuzhuyu ointment by HPLC

作　　者：周雪红[1] 李志浩[1] 

机构地区：[1]湖北医药学院附属东风医院药剂科,十堰442008

出　　处：《国际中医中药杂志》2011年第12期1101-1103,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的 建立同时测定复方吴茱萸软膏中吴茱萸碱和吴茱萸次碱含量的方法。方法 采用HPLC法，色谱柱为Agilent HC-C18（250 mm×4.6 mm，5 ？m），流动相为乙腈-0.08%辛烷磺酸钠（41∶59），流速为0.8 ml/min，柱温30℃，检测波长为225 nm。结果 吴茱萸碱和吴茱萸次碱分别在0.62～12.40 ？g/ml（r＝0.999 7）和0.24～4.80 ？g/ml（r＝0.9996）范围有良好的线性关系，平均加样回收率分别为97.9%、98.0%；RSD小于2.0%。结论 本方法快速简便，重复性好，可用于复方吴茱萸软膏的质量控制。Objective To establish a method for the determination of evodiamine and rutaecarpine of compound wuzhuyu ointment. Methods HPLC method was used with Agilent HC-C18（250 mm×4.6 mm, 5 ？m）column and the mobile phase being acetonitrile-0.08% ocane sulfonic acid sodium（41∶59）. The flow rate was 0.8 ml/min,the column temperature was 30℃, and the UV detector was set at 225 nm. Results The linear ranges of evodiamine and rutaecarpine were 0.62～12.40 ？g/ml（r=0.9997）and 0.24～4.8 0 ？g/ml（r=0.9996）, respectively. The average recovery of evodiamine and rutaecarpine was 97.9% and 98.0%, respectively. Conclusion The method is simple, repeatable and accurate. It can be applied in quantitative determination of evodiamine and rutaecarpine in compound Wuzhuyu ointment.

关 键 词：吴茱萸碱 吴茱萸次碱 复方吴茱萸软膏 高压液相色谱法 

分 类 号：R286.0[医药卫生—中药学]