苦马豆素对新城疫疫苗免疫效果的影响  

Effects of the Secondary Metabolites of Metarhizium anisopliae on Newcastle Disease Vaccine

作　　者：吴延磊[1] 杨鸣琦[1] 商育锦[1] 施珊珊[1] 吴小胜[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农业学报》2011年第8期25-29,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金项目(30270979)

摘　　要：研究金龟子绿僵菌次级代谢产物对鸡新城疫疫苗免疫效果的影响。将100只14日龄健康无免疫雏公鸡随机分为5组,第Ⅰ~Ⅲ组分别注射高、中、低（4、2、1 mg/kg）3种剂量的金龟子绿僵菌次级代谢产物;IV组和V组分别为无佐剂组对照组（No Adjuvant,NA）和空白对照组。第Ⅰ~IV组动物于14日龄以新城疫病毒（NDV）LaSota株弱毒活疫苗滴鼻进行第1次免疫,2周后加强免疫1次。分别于首免前和首免后连续6周在各免疫组随机抽取10只鸡检测血清中ND-HI抗体效价;于每次免疫后24 h检测外周血中白细胞数;于免疫后42日龄检测免疫器官指数;用MTT法检测脾脏淋巴细胞的增殖活性。金龟子绿僵菌次级代谢产物剂量为1~2 mg/kg,大多数试验组鸡的抗体效价与雏鸡脏器（胸腺,脾脏,法氏囊）指数及外周血中白细胞数都显著高于对照组。金龟子次级代谢产物单独或其协同脂多糖（LPS）,植物血凝素P（PHA-P）刺激时,鸡脾脏淋巴细胞的增殖活性增强。金龟子绿僵菌次级代谢产物剂量为4 mg/kg时,试验组鸡的抗体效价、免疫器官指数和外周血中的白细胞数均减少;鸡脾脏淋巴细胞的增殖活性降低。The trial was to study the effect of the secondary metabolites of Metarhizium anisopliae on newcastle disease vaccine.100 14-day-old chickens were randomly assigned into equal five groups.The chickens in three experimental groups were intramuscularly injected respectively with secondary metabolites of Metsrhizium anisopliae at high,medium,and low dose and vaccinated with Newcastle disease vaccine.Two control groups were administrated with no adjuvant（NA） and normal group.Four groups（group I to IV） were immunized with the LaSota live vaccine against Newcastle disease virus（NDV）for two times with 14 days interval.In continuous 6 weeks before and after vaccination,10 chickens were sampled randomly from each group for the determination of dynamic change of serum HI antibody;cellular numbers of leucocyte in peripheral blood were detected on the 24th day after vaccination,and 5 chickens per group were collected to weight whole body,spleen,bursa and thymus and calculated immune organ index on the 42nd day after vaccination.Proliferative response of spleen lymphocyte was examined by MTT method.The secondary metabolites of Metarhizium anisopliae at dosage of 1-4 mg/kg increased antibody titer,organ index and cellular number in blood,promoted the proliferative activity of spleen lymphocyte with secondary metabolites of Metsrhizium anisopliae alone or being combined with LPS and PHA-P.The cellular number in blood,organ index,antibody titer and the proliferative activity of lymphocyte were decreased at the dosage of 4 mg/kg.

关 键 词：金龟子绿僵菌 苦马豆素 抗体效价 脏器指数 脾淋巴细胞 

分 类 号：S858.31[农业科学—临床兽医学]