荧光标记鹿茸蛋白提取物胃肠道吸收离体实验研究  

In vitro study on gastrointestinal absorption of FITC labeled Pilose Antler protein extraction

作　　者：张倩[1,2] 胡剑江[1] 周秋丽[2] 王新月[2] 王毅[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]吉林大学药学院,吉林长春130021

出　　处：《药学学报》2011年第12期1526-1529,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30600842);中国中医科学院基本科研业务费自主选题(zx-zzxt-2006-005)

摘　　要：鹿茸是一种名贵的中药,含有丰富的蛋白质及多肽等活性成分,对神经、心血管、免疫、生殖系统有显著的调节作用[1]。鹿茸的传统给药方式为口服给药,给药后经消化道相关酶的降解发生一系列的变化,然而,由于检测方法的限制,An in vitro detection method of the gastrointestinal absorption of Pilose Antler protein was established for mixed protein activity.Five bands of protein with molecular weight of 17.8-160 kD derived from the Pilose Antler were extracted and sufficiently labeled with FITC（FITC-PE）.The stability and variation of FITC-PE in gastrointestinal circumstances were detected by native polyacrylamide gel electrophoresis and confocal laser scanning microscope.Results showed that the main component of FITC-PE kept invariant after being reacted with artificial gastric fluid and artificial intestinal fluid.The fluorescence signal was detected 20 min after administration in the valgus intestinal purse experiment,and three kinds of protein,with molecular weight of 45,25,and 17.8 kD,were detected in the mixture of absorbent protein.The research laid the foundation for the further in vivo study of Pilose Antler protein.Meanwhile,it would be an in vitro screening method for the absorption,distribution and metabolism of mixed protein from traditional Chinese medicine.

关 键 词：鹿茸蛋白提取物 荧光标记 人工胃液 人工肠液 肠囊外翻实验 

分 类 号：R965[医药卫生—药理学]