荷斯坦公牛PRNP多态性与精子活力相关性及抗病性评估  

Correlation analysis of PRNP polymorphisms to sperm motilityand disease resistance risk estimationin Chinese Holstein bull

作　　者：李杰[1] 张淑二 刘怡孝[1] 石国庆[3] 管峰[1,4] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018 [2]山东省畜牧总站,山东济南250022 [3]新疆兵团绵羊繁育生物技术重点实验室,新疆石河子832000 [4]中国计量学院植物环境生物学与检测技术研究所浙江省生物计量及检验检疫技术重点实验室,浙江杭州310018

出　　处：《中国预防兽医学报》2011年第12期940-944,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：农业部转基因生物新品种培育重大专项(2009ZX08008-001B);国家863计划项目(2011AA100307);国家自然科学基金(C120103)

摘　　要：为分析荷斯坦公牛朊蛋白基因(PRNP)多态性与精子活力的关系,并对其抗病性进行评估,选育抗病公牛,本实验以公牛精液为样品,研究PRNP基因中12 bp、23 bp和24 bp 3个片段的插入/缺失多样性、基因mRNA转录水平及其与精子活力等指标的关系。结果表明,12 bp和23 bp在群体中均得到3种基因型,24 bp只有2种基因型。不同基因型群体的mRNA转录水平存在显著差异,而基因型与精液产量和活力等指标存在相关性,抗病力和精子活力指标存在负相关。本研究中3个片段的插入-缺失多样性可以作为公牛选育的辅助标记。In this study, the polymorphisms of bovine prion protein gene （PRNP） and their relationship on production performances of sperm in Holstein bull were analyzed to select the disease resistant bull. The PRNP mRNA levels and its polymorphisms of 23 bp in-del, 12 bp in-del and 24 bp in-del were detected and their relationships on sperm motility were analyzed. Results showed that polymorphisms at the 23 bp and 12 bp exhibited 3 genotypes including in-in, in-del and del-del. While the polymorphisms at 24 bp fragment displayed 2 genotypes such as in-in and in-del. The PRNP mRNA transcript levels had significant difference between different genotype flocks, and the different levels had effects on sperm motility and ejaculation volume. However, it was also found that there was a negative relationship between the resistance and production performance in Holstein bull. The results in this study could be used as molecular markers for bull selection.

关 键 词：荷斯坦公牛 PRNP 多样性 精子活力 关联性 

分 类 号：S864.3[农业科学—野生动物驯养]