血清2型猪链球菌河南分离株毒力基因的鉴定及其cps2j全基因序列分析  

Identification of virulence genes and sequence analysis of cps2j gene of Streptococcus suis 2 isolated from Henan province

作　　者：张九州[1] 李乔晶[1] 郑逢梅[1] 常洪涛[1] 陈陆[1] 杨霞[1] 姚惠霞[1] 王川庆[1] 迪丽拜尔.阿木提 

机构地区：[1]河南农业大学禽病研究所,河南郑州450002 [2]新疆阿克苏职业技术学院,新疆阿克苏843000

出　　处：《中国预防兽医学报》2011年第12期982-984,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：河南省科技攻关项目(0624030030)

摘　　要：为了解血清2型猪链球菌(S.suis 2)河南分离株的毒力基因型及cps2j全基因突变情况,本研究扩增S.suis 2毒力基因gdh、cps2j、mrp、ef、sly、orf2和fbps,并对S.suis 2的cps2j进行全基因序列测定和同源性分析。结果表明,gdh、cps2j、mrp、ef、sly、orf2和fbps基因在8个S.suis 2分离株中的检出率分别为100%(8/8)、100%(8/8)、62.5%(5/8)、75%(6/8)、87.5%(7/8)、100%(8/8)和100%(8/8);其中cps2j全基因核苷酸及推导的氨基酸序列与来源不同的S.suis 2分离株同源性分别达98%和97%以上,以该基因构建的系统发育树表明8株S.suis 2河南分离株与国内外不同参考株同源性高,亲缘关系密切。To investigate virulence genes and mutation of cps2j gene of 8 Streptococcus suis serotype 2 isolated from Henan province, PCR methods for detecting 7 virulence genes of S. suis 2 were developed based on the specific primers for virulence genes of gdh, cps2j, mrp, el,, sly, oft2 and Pops. The results shown that the detection rate of gene gdh, cps2j, trup, el, sly, off2 and fbops were 100% （8/8）, 100% （8/8）, 62.5% （5/8）, 75% （6/8）, 87.5% （7/8）, 100% （8/8） and 100% （8/8）, respectively. The cps2j genes of 8 S. suis 2 isolates were over 98% and 97% homology for nucleotide and amino acid sequences with that of S. suis 2 isolates isolated from other areas, respectively. However, the high homology and close genetic relationship also found for cps2j gene of the 8 S. suis 2 isolates and reference strains isolated at home and abroad.

关 键 词：猪链球菌血清2型 毒力基因 序列分析 同源性 

分 类 号：S852.61[农业科学—基础兽医学]