铜对肉鸡原代肝细胞活性及糖原含量的影响  

The effect of copper on the activity of primary hepatic cells and the glycogen storage in broilers

作　　者：刘传敦[1] 苏荣胜[1] 颜诚[1] 朱华军[1,2] 李海琴[1] 唐兆新[1,2] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广东省兽用生物制品技术研究与应用企业重点实验室,广东肇庆510630

出　　处：《黑龙江畜牧兽医》2011年第11期1-3,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30671550;30871900)

摘　　要：为了探讨铜对肉鸡原代肝细胞活性及糖原含量的影响,试验分别用10,50,100μmol/L Cu2+在体外孵育原代肝细胞,PAS染色后测定30分钟和1,2,4,24,48,72小时各时间点的原代肝细胞活性及24小时时的糖原含量。结果表明:50μmol/L和100μmol/L Cu2+仅分别在72小时和4小时之后明显降低细胞活性;随着铜浓度的升高,糖原含量减少。说明高浓度铜可降低原代肝细胞活性,促进肝糖原分解。To investigate the effect of copper on the activity of primary hepatic cells and the glycogen storage,the primary hepatic cells in broilers were cultured in vitro with different concentrations of Cu2＋（10,50,100 μmol/L）,the activities of primary hepatic cells were measured at 30 min,1 h,2 h,4 h,24 h,48 h and 72 h respectively.The PAS staining was used to the glycogen storage at 24 h.The results showed that the activity of primary hepatic cells cultured with 50 and 100 μmol/L Cu2＋ was decreased significantly at 72h and 4h,correspondingly,the glycogen storage was reduced with the increase of Cu2＋ concentration.The results indicated that high level Cu2＋could reduce the activity of primary hepatic cells primarily and glycogen storage in broilers,promoting the decomposition of hepatic glycogen.

关 键 词：铜 原代肝细胞 糖原 含量 

分 类 号：S816.7[农业科学—饲料科学]