Direct Electrochemistry of Hemoglobin Based on Fe3O4@SiO2 Nanoparticles Modified Electrode  

作　　者：崔荣静 尹凡 周丽娟 潘宏程 

机构地区：[1]Jiangsu Laboratory of Advanced Functional Materials, Department of Chemistry and Materials Engineering Changshu Institute of Technology, Changshu, Jiangsu 215500, China [2]College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, Guangxi 541004, China

出　　处：《Chinese Journal of Chemistry》2011年第11期2481-2486,共6页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （Nos. 20905010, 20905016）, Jiangsu Provincial Natural Science Foundation （No. BK2008147）, Guangxi Natural Science Foundation （No. 0991082）, and China Postdoctoral Science Foundation （No. 20100471293）.

摘　　要：A biosensor based on hemoglobin-Fe304@SiO2 nanoparticle bioconjunctions modified indium-tin-oxide （Hb/Fe3O4@SiOz/ITO） electrode was fabricated to determine the concentration of H202. UV-vis absorption spectra, fourier transform infrared （FT-IR） spectroscopy, cyclic voltammetry （CV） and high-resolution transmission electron microscopy （HRTEM） were used to characterize the bioconjunction of Fe3O4@SiO2 with Hb. Experimental results demonstrate that the immobilized Hb on the Fe3O4@SiO2 matrix retained its native structure well. In addition, Fe3O4@SiO2 nanoparticles （NPs） are very effective in facilitating electron transfer of the immobilized enzyme, which can be attributed to the unique nanostructure and larger surface area of the Fe304@SiO2 NPs. The biosensor displayed good performance for the detection of H2O2 with a wide linear range from 2.03×10^-6 to 4.05×10^3 mol/L and a detection limit of 0.32 μmol/L. The resulting biosensor exhibited fast amperometric response, good stability, reproducibility, and selectivity to H2O2.A biosensor based on hemoglobin-Fe304@SiO2 nanoparticle bioconjunctions modified indium-tin-oxide （Hb/Fe3O4@SiOz/ITO） electrode was fabricated to determine the concentration of H202. UV-vis absorption spectra, fourier transform infrared （FT-IR） spectroscopy, cyclic voltammetry （CV） and high-resolution transmission electron microscopy （HRTEM） were used to characterize the bioconjunction of Fe3O4@SiO2 with Hb. Experimental results demonstrate that the immobilized Hb on the Fe3O4@SiO2 matrix retained its native structure well. In addition, Fe3O4@SiO2 nanoparticles （NPs） are very effective in facilitating electron transfer of the immobilized enzyme, which can be attributed to the unique nanostructure and larger surface area of the Fe304@SiO2 NPs. The biosensor displayed good performance for the detection of H2O2 with a wide linear range from 2.03×10^-6 to 4.05×10^3 mol/L and a detection limit of 0.32 μmol/L. The resulting biosensor exhibited fast amperometric response, good stability, reproducibility, and selectivity to H2O2.

关 键 词：Fe3O4@SiO2 nanoparticle BIOSENSOR HEMOGLOBIN direct electron transfer 

分 类 号：TQ633[化学工程—精细化工] TQ138.11