Release of the Oxygen-Evolving Complex Subunits from Photosystem II Membranes in Phosphorylation Condition under Light Stress  

作　　者：陈良兵 贾宏瑛 杜立波 田秋 高艳丽 刘扬 

机构地区：[1]State Key Lab for Structural Chemistry of Unstable and Stable Species, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China [2]Graduate University of the Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Chinese Journal of Chemistry》2011年第12期2631-2636,共6页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （Nos. 20875093 and 90813021） and the Pilot Project of Knowledge Innovation Program of the Chinese Academy of Sciences （No. KJCX2-SW-w29）.

摘　　要：So far it is unclear whether the release of oxygen-evolving complex （OEC） subunits including PsbO, PsbP, and PsbQ proteins is affected by the phosphorylation of photosystem II （PSII） membranes under light stress. In this work, different phosphorylated PSII membranes were obtained from spinach. Phosphorylation partially suppressed the release of PsbO, PsbP, and PsbQ proteins from PSII membranes under light stress. Reactive oxygen species including superoxide anion, hydrogen peroxide and hydroxyl radical, were involved in the release of a small part of PsbO protein, but not in the release of PsbP and PsbQ proteins in the non-phosphorylated and phosphorylated PSII membranes. All of the results suggested that the release of PsbO, PsbP, and PsbQ proteins was partially regulated by phosphorylation in PSII membranes, and the role of reactive oxygen species in the release of OEC subunits in non-phosphorylated PSII membranes was the same as in phosphorylated PSII membranes.So far it is unclear whether the release of oxygen-evolving complex （OEC） subunits including PsbO, PsbP, and PsbQ proteins is affected by the phosphorylation of photosystem II （PSII） membranes under light stress. In this work, different phosphorylated PSII membranes were obtained from spinach. Phosphorylation partially suppressed the release of PsbO, PsbP, and PsbQ proteins from PSII membranes under light stress. Reactive oxygen species including superoxide anion, hydrogen peroxide and hydroxyl radical, were involved in the release of a small part of PsbO protein, but not in the release of PsbP and PsbQ proteins in the non-phosphorylated and phosphorylated PSII membranes. All of the results suggested that the release of PsbO, PsbP, and PsbQ proteins was partially regulated by phosphorylation in PSII membranes, and the role of reactive oxygen species in the release of OEC subunits in non-phosphorylated PSII membranes was the same as in phosphorylated PSII membranes.

关 键 词：protein release reactive oxygen species EPR spectroscopy RADICALS photosystem II 

分 类 号：Q271[生物学—细胞生物学] S664.301[农业科学—果树学]