Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-Inositol,Vitexin,and Isovitexin Contents in Mung Bean  

作　　者：YAO Yang CHENG Xu-zhen REN Gui-xing 

机构地区：[1]Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

出　　处：《Agricultural Sciences in China》2011年第12期1986-1991,共6页中国农业科学（英文版）

基　　金：supported by the Talent Fund(to Dr. Ren Guixing)from the Chinese Academy of Agricultural Sciences;the Earmarked Fund for Modern Agro-Industry Technology Research System,China(nycytx-018)

摘　　要：Mung bean （Vigna radiata L.） is rich in D-chiro-inositol （DCI）, vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy （NIRS） was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography （HPLC）. The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares （PLS） algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions （R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin）. The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.Mung bean （Vigna radiata L.） is rich in D-chiro-inositol （DCI）, vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy （NIRS） was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography （HPLC）. The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares （PLS） algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions （R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin）. The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.

关 键 词：D-chiro-inositol  vitexin  isovitexin  NIRS  mung beanMun bean is an imoortant natural so 

分 类 号：TP311.132[自动化与计算机技术—计算机软件与理论] O657.33[自动化与计算机技术—计算机科学与技术]