基于特征荧光光谱的铜绿微囊藻MC-LR的快速预测  

Rapid Estimation on Microcystin-LR Concentration Released by Microcystic Aeruginosa Using Feature Fluorescence Spectroscopy

作　　者：段静波[1,2] 花日茂[1] 刘文清[2] 张玉钧[2] 王志刚[2] 肖雪[2] 王欢博[2] 

机构地区：[1]安徽农业大学资源与环境学院,安徽合肥230001 [2]中国科学院安徽光学精密机械研究所中国科学院环境光学与技术重点实验室,安徽合肥230031

出　　处：《大气与环境光学学报》2011年第6期443-449,共7页Journal of Atmospheric and Environmental Optics

基　　金：国家高技术研究发展计划(863计划)项目(2009AA063005);安徽省科技计划项目(08010301107);国家重大科技专项(2009ZX07527-005);安徽省自然科学基金(11040606M26)资助

摘　　要：以自然水体为培养基对铜绿微囊藻(Microcystic aeruginosa)进行了为期95天的实验室培养。测量了铜绿微囊藻不同生长阶段培养水体的三维荧光光谱和同步扫描荧光光谱,并且同步分析了藻类叶绿素a浓度和胞外微囊藻毒素MC-LR浓度.结果表明:根据三维荧光光谱得到荧光信号A、信号X和信号C,分别代表类蛋白、海洋腐殖酸和类富里酸.对各荧光信号强度与细胞外微囊藻毒素MC-LR浓度以叶绿素a浓度进行归一化,发现二者在藻类衰亡期具有相同的变化趋势,且最大值出现的时间较为一致,两者表现出显著的线性相关性.因此,结合荧光光谱特征信号和叶绿素a浓度可以实现对藻毒素MC-LR浓度的快速预测.就实验方法而言,Δλ=80 nm的同步扫描荧光光谱法相对于三维荧光光谱法具有耗时少、测量简便等优点,可以完整反映荧光信号A、X和C的强度,更适合用于以藻毒素快速预测为目的的水体荧光光谱在线分析.Microcystic aeruginosa was cultivated in laboratory condition with nature water for about 95 days. Three dimensional excitation-emission（3DEEM） fluorescence spectroscopy and synchronous scan fluorescence spectroscopy of the culture water at different growth stages were measured,simultaneously the chlorophyll-a and extracellular MC-LR concentration were analyzed.The results show that fluorescence signal A,signal X and signal C could be observed obviously from 3DEEM fluorescence spectroscopy and synchronous scan fluorescence spectroscopy,which represent protein-like substance,marine humic-like acid and fulvic-like substance respectively.All the three fluorescence signals intensity and MC-LR concentration were normalized by chlorophyll-a concentration.In the decline phase,the normalized fluorescence signals（A,X,C） and normalized MC-LR concentration show the same dynamic laws and good linear correlation.The maximum values appeared at the same time.Therefore,MC-LR concentration can be estimated by the feature fluorescence signal（A, X,C） and chlorophyll-a concentration.Comparing to 3DEEM fluorescence spectroscopy,synchronous scans fluorescence spectroscopy（△λ=80 nm） is less time-consuming and can give out the entire feature fluorescence signal（A,X,C）.It is suitable for real-time analysis of water fluorescent spectroscopy which can be used to rapidly estimate the MC-LR concentration.

关 键 词：微囊藻毒素 三维荧光光谱 同步扫描荧光光谱 

分 类 号：O433[机械工程—光学工程] X832[理学—光学]