太阳扇愈伤组织培养及植株再生体系建立  

Callus Culture of Scaevola aemula and Establishment of a Highly Efficient System for Its Plantlet Regeneration

作　　者：于传[1] 何丙辉[1] 刘玉民[1] 蔡兴华[1] 李薇[1] 

机构地区：[1]西南大学资源环境学院三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2011年第11期12-17,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家林业局"948"项目资助(2006-4-88);重庆市自然科学基金重点资助项目(CSTC-2008BA7032);西南大学生态学重点学科"211工程"建设经费资助

摘　　要：以太阳扇(Scaevola aemula)嫩叶、老叶、茎段、茎节为材料进行外植体筛选;以太阳扇嫩叶为外植体,研究愈伤组织诱导、不定芽分化及增殖、生根培养的适宜条件.结果表明:嫩叶为太阳扇愈伤组织诱导最佳外植体;MS+6-BA 0.50mg/L+2,4-D 0.20mg/L为愈伤组织诱导最佳培养基;MS+6-BA 0.50mg/L+NAA 0.10mg/L为芽分化最佳培养基,分化率可达96.5%;1/2MS+NAA 0.01mg/L为芽增殖最佳培养基,增殖系数最高可达3.33;糖浓度、NAA均影响太阳扇无菌苗生根,1/2MS+NAA 0.02mg/L+蔗糖30g/L为生根培养最适培养基;g腐殖质∶g珍珠岩∶g细土=2∶1∶1为组培苗移栽最佳基质,移栽成活率达75%.The young leaf,old leaf,stem segment and stem node of Sunfan（Scaevola aemula） were used in in vitro culture.The young leaf was shown to be the best explant and,therefore,was cultured to identify the optimum conditions for callus induction,adventitious bud differentiation and plantlet regeneration and rooting of this species.The results showed that the best media were MS＋6-BA 0.50 mg/L＋2,4-D 0.20 mg/L for callus induction,MS＋6-BA 0.50 mg/L＋NAA 0.10 mg/L for adventitious bud differentiation（the differentiation rate being as high as 96.5%）,1/2MS＋NAA 0.01 mg/L for bud proliferation with a proliferation rate of 3.33 and 1/2MS＋NAA 0.02 mg/L＋ sucrose 30 g/L for the rooting of the regenerated plantlets.The matrix with a humus∶perlite∶fine soil ratio of 2∶1∶1 was suitable for in vitro plantlet transplantation and a survival rate of ≥75% of the transplanted plants was achieved.

关 键 词：太阳扇 组织培养 植株再生 

分 类 号：S682.39[农业科学—观赏园艺]