超声提取-离子色谱法测定蜂蜜柚子茶中的可溶性糖  

Determination of Soluble Sugars in Honey Pomelo Tea by Anion Chromatography with Ultrasonic Extraction

作　　者：许丽[1] 周光明[2] 余娜[1] 张丽贤[1] 

机构地区：[1]发光与实时分析教育部重点实验室,重庆400715 [2]西南大学化学化工学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2011年第11期90-94,共5页Journal of Southwest University(Natural Science Edition)

基　　金：发光与实时分析重庆市重点实验室(西南大学)资助项目(CSTC2006CA8006);重庆市自然科学基金资助项目(CSTC2007BB5370);科技部重大专项资助项目(2008ZX07315)

摘　　要：建立超声提取-高效阴离子交换-脉冲安培检测(HPAE-PAD)同时分离并测定蜂蜜柚子茶中可溶性糖的分析方法.采用METROSEP CARB 1(150mm×4.0mm)分离柱,对分离条件进行了优化,确定淋洗液为5.0mmol/L的NaOH溶液,流速为1.0mL/min.蔗糖、葡萄糖、果糖的检出限分别为0.2,0.1,1.0μg/mL.混合标准溶液连续6次进样,峰高相对标准偏差为1.56%3.40%,回收率为86.80%98.80%.该方法样品前处理简单,灵敏度高,为蜂蜜柚子茶的质量检测提供了一个简单有效的方法.A method for the determination of soluble sugars in honey pomelo tea by high performance anion exchange chromatography with ultrasonic extraction and pulsed amperometric detection（HPAE-PAD） is described in this paper.The separation conditions were investigated on METROSEP CARB 1（150 mm×4.0 mm） column.The optimal separations were achieved with 5.0 mmol/L NaOH as the eluent at a rate of 1.0 mL/min.The detection limits of sucrose,glucose and fructose were 0.2 μg/mL,0.1 μg/mL and 1.0 μg/mL,respectively.The RSDs（relative standard deviation） of peak height for six consecutive determinations varied from 1.56% to 3.40%,with a recovery of 86.80%-98.80%.The above method proved to be simple and highly sensitive for sample pretreatment and is,therefore,recommended for quality inspection of honey pomelo tea.

关 键 词：蜂蜜柚子茶 可溶性糖 离子色谱 脉冲安培检测 

分 类 号：O657.75[理学—分析化学]