基于纳米金在线扫集-毛细管电泳法测定尿样中8-羟基-2′脱氧鸟嘌呤核苷  

CE Determination of 8-Hydroxy-2′-deoxguanosine in Urine Samples Based on Enrichment by Online Sweeping with Nanogold

作　　者：颜流水[1] 任艳[1] 郭会琴[1] 熊萌瑶[1] 黄燕[1] 薜昆鹏[1] 

机构地区：[1]南昌航空大学环境与化学工程学院,南昌330063

出　　处：《理化检验（化学分册）》2011年第11期1253-1255,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金资助项目(20765003)

摘　　要：提出了纳米金在线富集-毛细管电泳法测定尿样中8-羟基-2′脱氧鸟嘌呤核苷(8-OHdG)的方法。试验选择了以下的分析条件:①运行电压-20kV;②背景电解质为pH 8.2的20mmol.L-1硼砂(含粒径10nm纳米金溶液200μL和0.1mmol.L-1 CTMAB);③检测波长254nm。试验证明背景电解质中的纳米金粒子与CTMAB形成胶束,提高了对8-OHdG的扫集能力,8-OHdG与dG在10min内可实现基线分离。8-OHdG浓度在0.50～50.0μmol.L-1范围内呈线性,检出限(3S/N)为39nmol.L-1。方法用于尿样中8-OHdG含量的测定,所得加标回收率在90.0%～104.6%之间。A method for the determination of 8-hydroxy-2′-deoxguanosine（8-OHdG） in urine sample by CE based on enrichment by online sweeping with nanogold was proposed.Analytical conditions were studied and following optimized parameters were given： ① moving voltage：-20 kV;② electrophoretic medium： 20 mmol·L-1 of borax solution（pH 8.2,including 10 nm nanogold solution of 200 μL and 0.1 mmol·L-1 CTMAB;③ wavelength of detection： 254 nm.It was shown that micelle was formed between nanogold and CTMAB in the electrophoretic medium,which conduced to the sweeping of 8-OHdG.The baseline resolution was attained within 10 min.Linearity range between 0.50 and 50.0 μmol·L-1 was obtained with detection limit（3S/N） of 39 nmol·L-1.The proposed method was used in the determination of 8-OHdG in a urine samples,gving recovery found by standard addition method in the range of 90.0%-104.6%.

关 键 词：毛细管电泳法 在线扫集 8-羟基-2′脱氧鸟嘌呤核苷 纳米金 

分 类 号：O657.8[理学—分析化学]