核黄素在镀铂铅笔芯热电极上的电致化学发光检测  

Determination of Riboflavin by Electrochemiluminescence at Platinum Coated Heated Pencil Graphite Electrode

作　　者：吴爱红[1] 孙建军[1] 吴韶华[1] 

机构地区：[1]福州大学食品安全分析与检测教育部重点实验室,福州大学化学化工学院,福州350108

出　　处：《分析化学》2011年第12期1898-1902,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.20775015和20975022)资助项目

摘　　要：利用电沉积方法制作了镀铂铅笔芯热电极(Pt/HPGE),用循环伏安法(CV)及扫描电镜(SEM)对其性质进行了表征。此电极具有价廉易得、重现性好及温度响应灵敏等特点。在此电极上研究了核黄素(RF)对Ru(bpy)32+-C2O42-电致化学发光体系的猝灭作用,并推测了可能的机理,据此建立了灵敏检测RF的新方法。电极温度升高到58℃时,检出限可达1.9×10-10mol/L(S/N=3),比室温时明显降低。应用于维生素药片中RF含量的测定,平均回收率为102.8%。A platinum coated heated pencil graphite electrode（Pt/HPGE） was fabricated and its properties were characterized by cyclic voltammetry（CV） and scanning electron microscopy（SEM）,etc.It was demonstrated that Pt/HPGE had unique properties such as cheapness,easy preparation,good reproducibility and sensitive temperature response.The Pt/HPGE was employed to detect riboflavin（RF） sensitively based on the quenching effect of RF on Ru（bpy）32＋/C2O42-electrochemiluminescence（ECL） system.The limit of detection was 1.9×10-10 mol/L（S/N=3） at an electrode temperature of 58 ℃,which clearly lower than that at room temperature.The method was applied to the determination of RF in vitamin pills with an average recovery of 102.8%.

关 键 词：镀铂 铅笔芯 热电极 核黄素 电致化学发光 

分 类 号：R284.1[医药卫生—中药学] O657.3[医药卫生—中医学]