种子细胞的生长时相和紫杉醇生产的关系研究  

The Relationship between Taxol Production and Inocula from Different Periodsof the Growth Cycle in Taxus Chinensis Suspension Cultures

作　　者：张长河[1] 梅兴国[1] 余龙江[1] 

机构地区：[1]华中理工大学生命科学与技术学院

出　　处：《华中理工大学学报》1999年第A01期64-66,共3页Journal of Huazhong University of Science and Technology

基　　金：国家科技部"八五"( 85-08-06-02 )和"九五"( 96-C02-03-01)重点科技攻关资助项目

摘　　要：处在生长周期不同时相的红豆杉细胞作为种子细胞,接入含来自红豆杉树皮的真菌诱导子的生产培养基中,紫杉醇的生产和种子细胞的生长时相有明显的对应关系.来自对数生长后期和稳定前期的细胞,胞内紫杉醇含量分别为26.8μg·g-1和58.1μg·g-1;来自稳定后期的细胞对诱导子反应最敏感,紫杉醇含量比来自延迟期和对数生长初期的细胞中的紫杉醇含量提高了两个数量级,达421μg·g-1.The relationship between taxolproduction and inocula wasinvestigated from differentperi ods of the growth cycle of Taxus chinensis suspension cultures in the production medium ( modified MS) with fungalelicitors derived from the bark of T.chinensis.Taxolconcentration was2 6.8μg· g- 1and5 8.1 μg· g- 1in culturesstarted with cellsfrom thelate exponentialphaseand early stationary phase,respectively.Taxolproduction was about1 0 0 fold higher in cultures started with cells from the late stationary phase than thatin cultures started with cells from the lag and exponentialphase, up to 4 2 1μg· g- 1.

关 键 词：红豆杉 细胞培养 紫杉醇 种子细胞 生长时相 

分 类 号：S791.49[农业科学—林木遗传育种] Q81[农业科学—林学]