婴儿双歧杆菌介导的sFlt-1真核表达系统对Lewis肺癌小鼠的抑瘤效果  

Anti-tumor effect of Bifidobacterium infantis-mediated sFlt-1 eukaryotic expression system on Lewis lung cancer in mice

作　　者：邓丽聪[1] 许士叶[2] 毛淑华[3] 易成[4] 黄英[1] 

机构地区：[1]四川大学华西基础医学与法医学院,四川成都610041 [2]重庆医科大学基础医学院,重庆400042 [3]巴中市中心医院肿瘤科,四川巴中636000 [4]四川大学华西医院腹部肿瘤科,四川成都610041

出　　处：《华西药学杂志》2011年第6期564-566,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:30570693);四川省科技厅攻关计划科研基金(2006Z09-030)

摘　　要：目的观察婴儿双歧杆菌介导的sFlt-1真核表达系统对Lewis肺癌(LLC)小鼠的抑瘤效果。方法将24只C57BL/6LLC小鼠随机分为pcDNA3.1婴儿双歧杆菌组(A组)、pcDNA3.1-sFlt-1重组婴儿双歧杆菌组(B组)及生理盐水对照组(C组)。观察小鼠的生存质量、肿瘤体积、肿瘤重量及抑瘤率,并取血、肿瘤组织及其他组织进行厌氧培养;另取45只荷瘤鼠观察生存率。结果 B组小鼠的肿瘤体积及重量明显小于A、C组,抑瘤率明显高于A组;A、B组的肿瘤组织厌氧培养时可见大量白色菌落生长,其他组织未见菌落生长,血培养仅见几个菌落生长。B组小鼠的存活率及生存质量明显优于A、C组。结论婴儿双歧杆菌介导的sFlt-1真核表达系统可明显抑制肿瘤生长、显著提高荷瘤鼠的存活率,具有良好的安全性及肿瘤靶向性。OBJECTIVE To observe the anti-tumor effect of Bifidobacterium infantis-mediated sFlt-1 eukaryotic expression system on the Lewis lung cancer（LLC） in mice as well as its safety and tumor-targeting effect.METHODS 24 C57BL/6 mice implanted with LLC were randomized into the pcDNA3.1 Bifidobacterium infantis group（group A）,pcDNA3.1-sFlt-1 recombinant Bifidobacterium infantis group（group B） and normal saline control group（group C）.The quality of mice of life（QOF）,tumor volume,tumor weight and inhibitive rate of tumor were observed.Blood,tumor tissues and the other tissues were cultured under anaerobic environment.In addition,the survival rate of another 45 tumor-bearing mice was observed.RESULTS The tumor volume and weight in group B were much smaller than those of group A and group C,and the inhibition rate of tumor in group B was higher than that in the group A.In addition,lots of white bacteria colonies of tumor tissues grew in group A and B under anaerobic culture,whereas the other tissues showed no bacteria colony,only a few bacteria colonies formed on blood culture in group A and B.The cumulative survival rate and QOF of mice in group B were significantly improved than these of the group A and group C.CONCLUSION The Bifidobacterium infantis-mediated sFlt-1 eukaryotic expression system could not only significantly inhibit the tumor growth and prolong survival time of tumor-bearing mice,but also had good security and tumor targeting effect.

关 键 词：SFLT-1 婴儿双歧杆菌 抑瘤 抗血管生成 肿瘤靶向 

分 类 号：R96[医药卫生—药理学]