基于碳纳米管固定葡萄糖氧化酶的ECL葡萄糖传感器的制备  

Fabrication of ECL glucose sensor based on immobilization glucose oxidase on carbon nanotubes modified electrode

作　　者：张然[1] 杨善丽[1] 买楠楠[1] 魏万之[1] 罗胜联[1] 聂舟[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《传感器与微系统》2011年第12期98-101,共4页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(50725825)

摘　　要：将葡萄糖氧化酶(GOD)固定在多壁碳纳米管(MWCNTs)修饰电极(ME)上,GOD催化氧化葡萄糖生成过氧化氢,并使鲁米诺产生电致化学发光(ECL),据此构建了一种新型ECL葡萄糖传感器。结果表明:CNTs修饰的电极对鲁米诺和H2O2反应具有显著的电催化活性和增敏效果。该传感器对葡萄糖检测的线性范围为0.01～10.0mmol/L,相关系数R=0.999(n=5),检测限为5.0μmol/L。此传感器响应快,稳定性高,测定条件接近人体自然pH。将所建立的方法用于临床血清样品中葡萄糖含量的测定,获得了满意的结果。Incorporation of muhi-walled carbon nanotubes on the electrode is performed and modification of glassy carbon electrode with the prepared nano-hybrid material led to the fabrication of a novel electrode. The modified electrode shows attractive electrocatalytic activity and sensitizing effect on Luminol-H2O2 electrochemiluminescence （ECL） reactions at neutral media. The sensitized Luminol-H2O2 reactions are successfully applied for the ECL determination of glucose. Under the optimal conditions for Luminol-H2O2 system, the ECL signal intensity of luminol is linear with the concentration of glucose in the range of 0.01 -10.0 mmol/L,correlation coefficient R = 0. 999 （ n =5 ）, the limits of detection for glucose are 5.0μmol/L. Excellent electrocatalytic activity, high stability, technically simple and possibility of preparation at short period of time are of great advantages of this glucose biosensor.

关 键 词：多壁碳纳米管 修饰电极 电致化学发光 葡萄糖氧化酶 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]