培氟沙星人工抗原的合成与鉴定  

Synthesis and identification of Pefloxacin artificial antigens

作　　者：孙薇薇[1] 上官陶[1] 俞亚玲[1] 张冠世[1] 李引乾[1] 李麟[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2011年第12期26-30,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学大学生科技创新项目

摘　　要：【目的】制备培氟沙星(Pefloxacin,PEF)完全抗原,并对其免疫学特性进行鉴定。【方法】以培氟沙星与牛血清白蛋白(BSA)为材料,用碳二亚胺(EDC)法制备培氟沙星-牛血清白蛋白完全抗原(PEF-BSA),变性聚丙烯酰胺凝胶电泳(SDS-PAGE)及紫外扫描(UV)检测其免疫学特性;利用PEF-BSA免疫BALB/c小鼠,无菌条件下收集血清,用间接ELISA测定多抗血清(pAb)效价。并用同样的方法制备和鉴定培氟沙星-鸡卵清白蛋白完全抗原(PEF-OVA)。【结果】培氟沙星与牛血清白蛋白及鸡卵清白蛋白偶联成功,PEF-BSA偶联比为8∶1,PEF-OVA偶联比为6∶1。间接ELISA测得多抗血清效价均达到4 000以上,得到了较好的免疫效果。【结论】成功得到了培氟沙星完全抗原,该抗原具有较强的特异性及良好的敏感性。【Objective】 The study was done in order to prepare Pefloxacin（PEF） artifical antigen and identify its immunological characteristics.【Method】 Using EDC,the PEF was coupled with BSA（OVA）to prepare artifical antigen（PEF-BSA）.Ultraviolet scanning and SDS-Polyacrylamide gel electrophoresis were applied to explore its immunological characteristics.BALB/c mice were immunized with PEF-BSA,the serum was collected under aseptic conditions,and the polyclonal antibody serum（pAb） was identified by ELISA.【Result】 The result showed that PEF-BSA and PEF-OVA were synthesized successfully and the conjugation ratio of PEF to BSA was about 8∶1,PEF to OVA 6∶1.Both of the pAb were above 4 000.【Conclusion】 PEF artifical antigen was prepared,and the results indicated that artifical antigen had a favorable sensitivity and specificity.

关 键 词：培氟沙星 人工抗原 碳二亚胺法 ELISA检测 

分 类 号：S852.43[农业科学—基础兽医学]