外源CNT对哈白兔体内CNT和GH及瘦素动态变化的影响  

Exogenous CNT regulation research on dynamic variation of blood fat,sugar and protein in Haerbin white rabbits

作　　者：刘永峰[1,2] 昝林森[1] 刘登科[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]陕西师范大学食品工程与营养科学学院,陕西西安710062

出　　处：《西北农林科技大学学报（自然科学版）》2011年第12期60-66,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30871788);教育部"长江学者和创新团队发展计划"项目(IRT0940);陕西师范大学青年科技项目(201001009);中央高校基本科研业务费专项(GK201002040)

摘　　要：【目的】研究外源性环核苷酸(CNT)对动物血浆中内源环鸟苷酸(cGMP)和环腺苷酸(cAMP)、生长激素(GH)及瘦素动态变化规律的调控作用。【方法】以哈白兔为试验动物,设置对照组(注射生理盐水)、cGMP组、cAMP组和cGMP+cAMP组,后3个处理组分别外源注射cGMP、cAMP及其等量混合物的腹腔注射液,在注射后1,3,6,10,15,21,28,36,45,55,65,75,85,95h采集血样,分别运用放射免疫法测定动物内源cGMP、cAMP、瘦素及GH的含量。【结果】单独注射cGMP可以显著提高内源性cGMP的水平,而且在注射后45h以内效果最佳;单独注射cAMP可以显著提高内源性cAMP的水平,而且在4d或者更长时间会持续有效;无论单独注射cAMP或cGMP,还是混合注射cAMP+cGMP,内源性cAMP总有优于内源性cGMP 6%的优势。此外,cGMP对血浆瘦素水平有较小的正调控作用,cAMP对血浆瘦素水平有负调控作用,cGMP+cAMP对血浆瘦素的调控作用介于以上两者之间,而且cAMP的作用相对较为明显;注射cAMP后的55h以内,瘦素质量浓度明显降低,以后趋于正常;cGMP、cAMP及cGMP+cAMP对血浆GH质量浓度均有显著的正向调控作用,而且以cGMP的优势更为明显。【结论】探讨了外源CNT作用下内源cGMP、cAMP、瘦素及GH的动态变化规律,为通过外源CNT来调节动物体生理水平提供了理论依据。【Objective】 The aim of this study was to find out exogenous CNT＇s regulation effect on the dynamic variation law of endogenous CNT,Leptin and GH in animals.【Method】 In this study,Haerbin white rabbits were selected as object,and four different treatment groups：control group,cGMP group,cAMP group and cGMP＋cAMP group,were set in the experiment.Then we exogenously injected cGMP,cAMP and cGMP＋cAMP（1∶1） into rabbits＇blood and took blood samples 1,3,6,10,15,21,28,36,45,55,65,75,85 and 95 h later.Finally,Radio-immunity method was used to determine the content of CNT,Leptin and GH in different treatment groups.【Result】 Injecting cGMP alone could increase endogenous cGMP significantly,and the effect was optimum after 45 h of injection.cAMP injection could also increase endogenous cAMP,and the effect was durative in 4 d or later.Both injecting CNT alone and injecting the cAMP＋cGMP,cAMP level was 6% higher than injecting cGMP.Furthermore,cGMP had a little positive effect on Leptin,but cAMP had obvious negative effect.cGMP＋cAMP had a middle effect between cGMP and cAMP.Leptin content deceased obviously 55 h within injecting cAMP,then it went back to normal.In addition,cGMP,cAMP,cGMP＋cAMP had significant positive effect on GH,and cGMP had more obvious effect.【Conclusion】 Dynamic variation laws of cGMP,cAMP,Leptin and GH provide beneficial theoretical basis in regulating and controlling animal physiological level using exogenous CNT.

关 键 词：环核苷酸 哈白兔 瘦素 生长激素 

分 类 号：S816.73[农业科学—饲料科学]