邻苯二甲酸二乙基己基酯对大鼠睾丸细胞DNA损伤和睾丸组织氧化应激影响  

Study on DNA damage and oxidative stress in testicular tissue of rats induced by di-(2-ethylhexyl) phthalate

作　　者：蔡红平[1] 肖雯[2] 朱静[3] 衷筱琴[2] 

机构地区：[1]南昌大学公共卫生学院环境卫生教研室,江西南昌330006 [2]南昌大学基础医学院病理教研室,330006 [3]南昌大学医学院实验教学部,330006

出　　处：《中国职业医学》2011年第6期458-462,共5页China Occupational Medicine

基　　金：江西省科技支撑计划项目(2009JX00870);南昌大学科研课题(2008018)

摘　　要：目的探讨邻苯二甲酸二乙基己基酯(DEHP)对大鼠睾丸细胞DNA的损伤和睾丸组织氧化应激的作用。方法雄性Wistar大鼠25只,随机分为5组,每组5只,通过灌胃的方法摄入DEHP,4个染毒组剂量分别为1 500、3 000、4 500、6 000 mg.kg-1.d-1,1个阴性对照组。用单细胞凝胶电泳技术检测大鼠睾丸细胞DNA的损伤,睾丸组织中活性氧(ROS)、丙二醛(MDA)的水平以及睾丸组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活力。结果各染毒组和阴性对照组大鼠睾丸细胞DNA损伤率分别为29.0%、71.0%、89.0%、95.0%和15.0%,各染毒组睾丸细胞DNA损伤程度与对照组比较,差异均有统计学意义(P<0.05,P<0.01),且大鼠睾丸细胞DNA损伤率与染毒剂量之间呈剂量-反应关系(r=0.930,P<0.01);各染毒组大鼠睾丸组织的ROS、MDA的水平均较阴性对照组显著升高(P<0.05,P<0.01),而GSH-Px、SOD的活力又较阴性对照组明显下降(P<0.05,P<0.01) DEHP可造成大鼠睾丸组织明显的氧化应激并引起睾丸细胞DNA的损伤。Objective To study the effect of di-（2-ethylhexyl） phthalate （DEHP） on DNA damage and oxidative stress in testicular tissue of rats. Methods Wistar male rats were divided into 5 groups, including 4 experimental groups, which were re- spectively exposed to 1 500, 3 000, 4 500 and 6 000 mg ·kg^-1· d ^-1 DEHP, and a control group. The degree of DNA damage in rat testicle cells was detected by single cell gel electrophoresis. Detected were the contents of reactive oxygen species （ROS） , malondialdehyde （MDA） in testicular tissue, and the activities of glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD） in testicular tissue. Results The rates of testicle cell l）NA damage in each experimental groups and the control group were 29. 0% ,71.0% ,89. 0% ,95.0% ,and 15.0% respectively, showing DNA damage in all experimental groups were signifi- cantly higher than those in the control （P 〈 0.05,P 〈 0. 01 ）. There was a significant dose-response relation between the rates of testicle cell l）NA damage and the doses of DEHP （ r = 0. 930, P 〈 0. 01 ）. The contents of ROS and MDA of 4 experimental groups increased significantly compared with those of the control （P 〈0. 05 ,P 〈0. 01 ）. The activities of GSH-Px and SOD of 4 experimental groups reduced significantly compared with those of the control （P 〈 0. 05, P 〈 0. 01 ）. Conclusion DEHP could cause oxidative stress and DNA damage in testis spermatogone of rats.

关 键 词：邻苯二甲酸二乙基己基酯 DNA损伤 氧化应激 睾丸 

分 类 号：R114[医药卫生—卫生毒理学] R994.6[医药卫生—公共卫生与预防医学]