斑点金免疫渗滤法测定人全血中抗HBc抗体  被引量:3

Detecting anti-HBc antibody in whole blood by dot immunogold filitration assay

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作  者:邓为民[1] 徐世文[1] 潘菊芬[1] 

机构地区:[1]天津医科大学免疫学教研室,天津300070

出  处:《中国免疫学杂志》1999年第12期572-573,共2页Chinese Journal of Immunology

摘  要:目的:建立一种适合斑点金免疫渗滤法(Dot Immu no goldFiltrationAssayDIGFA)特点的快速全血诊断方法。方法:选择可瞬间分离血中细胞和血清的膜,依据DIGFA的原理对33份HBcAb阳性血样和48份阴性血样进行了全血抗HBcAg抗体的检测。结果:测定阳性标本符合率为96.7%,阴性标本符合率为100%。结论:提供了一种简便、快速、可行的全血检测抗HBcAg抗体的方法。Objective:TO establish a rapid, simple and reliable assay for detecting anti-HBc Ab in human whole blood.Methods: A newdot immunogold filtration assay was selected. Result: In detecting 33 anti-HBc Ah positive samples and 48 negative samples confirmed by clinical indexes, the positive coincidence rate was 96.7%; and negative late was 100% Conclusion:This method not only has the characteristicsof rapidness,simpleness and reliability, but the has an obvious advantage, that we could detect the anti-HBc Ab in whole bind, but not inserum, and obtain the result immediately.

关 键 词:抗HBC Ab 全血 DIGFA 

分 类 号:R446.62[医药卫生—诊断学] R512.620.4[医药卫生—临床医学]

 

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