伴刀豆球蛋白ConA体外诱导口腔鳞癌细胞Tca8113凋亡的机制初探  

Concanavalin A induces apoptosis in human oral squamous carcinoma Tca8113 cells and its molecular mechanisms

作　　者：张鹏[1] 金辉[2] 彭沥[2] 鲍锦库[3] 

机构地区：[1]首都医科大学附属北京儿童医院口腔科,北京100045 [2]成都军区联勤部门诊部,成都610015 [3]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2011年第6期1421-1426,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30970643)

摘　　要：分别用0μg/mL到100μg/mL的伴刀豆球蛋白ConA加入人口腔鳞癌Tca8113细胞,采用MTT法检测ConA对Tca8113细胞的增殖抑制作用,并利用形态学观察、乳酸脱氢酶活力测定法(LDH)和流式细胞仪测定细胞凋亡,最后进行了caspase活性检测.加入25μg/mL的ConA培养24 h后,细胞的生长抑制率接近了50%.形态学观察检测到了明显的凋亡的形态学特征,如细胞皱缩、细胞质细胞核凝集等.乳酸脱氢酶活力测定法和流式细胞仪检测显示口腔鳞癌细胞凋亡率随ConA浓度升高而增加.Caspase活力检测显示ConA诱导Tca8113细胞死亡是通过caspase依赖的途径来诱导细胞凋亡.伴刀豆球蛋白ConA对人口腔鳞癌细胞Tca8113的增殖具有显著的抑制作用,并可以通过时间剂量依赖的方式,通过caspase依赖的途径诱导细胞凋亡.ConA from 0μg/mL to 100 μg/mL was added into Tca8113 cells, and MTT, cellular morphology observation, LDH assay, flow cytometry detection and caspase assay were performed. MTT was applied to detect the inhibitory ratio of Tca8113 cells, and. morphologic changes, LDH, flow cytometry detection were applied to detect apoptosis. In addition, caspase assy was also applied to observe whether caspase was involved in ConA-induced apoptosis in Tca8113 cells. After 24 h incubation with 25μg/mL ConA, the inhibitory rate reached nearly 50%. Typical morphologic changes like membrane blebbing, chromatin condensation and nuclei fragmentation were confirmed. LDH assay and flow cytom- etry detection showed that with the progressively increasing the concentration of ConA, more and more apoptotic cells appeared. And, caspase assay demonstrated that ConA could induce apoptosis in Tca8113 cells in a caspase-dependent pathway. In conclusion, ConA induces apoptosis in human oral squamous carcinoma TcaS113 cells by caspase-mediated pathway in a time- and dose-dependent manner.

关 键 词：伴刀豆球蛋白(ConA) 口腔鳞癌 凋亡 

分 类 号：Q51[生物学—生物化学]