MMP-9/IgA和TIMP-1/IgA在IgA肾病患者肾组织中的共表达及意义  

Co-expression of MMP-9/IgA and TIMP-1/IgA in renal tissue of IgA nephropathy and its significance

作　　者：师锁柱[1] 张雪光[1] 陈香美[1] 

机构地区：[1]解放军总医院肾病科肾脏病国家重点学科暨国家重点实验室,北京100853

出　　处：《军医进修学院学报》2011年第12期1269-1270,1273,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的观察基质金属蛋白酶-9(MMP-9)及其组织抑制因子-1(TIMP-1)和免疫球蛋白A(IgA)在IgA肾病不同病变肾小球内共表达情况,探讨IgA沉积在IgA肾病进展中的作用。方法采用石蜡切片免疫荧光双标记技术,对56例不同病理分级IgA肾病(Lee氏分级Ⅰ-Ⅴ级)患者肾组织中MMP-9/IgA、TIMP-1/IgA共表达变化进行检测。结果在Lee氏分级Ⅰ-Ⅱ级肾小球中TIMP-1/IgA有少量表达,MMP-9/IgA表达最多。Lee氏分级Ⅲ级肾小球中TIMP-1/IgA表达增多,MMP-9/IgA表达减少,在Lee氏分级Ⅳ-Ⅴ级重度系膜病变肾小球内TIMP-1/IgA表达最多,MMP-9/IgA有少量表达,而在硬化肾小球内两者表达最少。结论在IgA肾病进展过程中随着肾小球内IgA沉积增多,肾小球内TIMP-1表达逐渐增高,MMP-9表达逐渐减少,提示IgA沉积引起的MMP-9/TIMP-1失衡参与了IgA肾病肾小球硬化过程。Objective To study the role of immunoglobulin A（IgA） deposition in the progress of IgA nephropathy by observing the co-expression of matrix metalloproteinase-9（MMP-9）,its tissue inhibitor-1（TIMP-1） and IgA in IgA nephropathy patients with variant glomerular lesions.Methods Co-expression of MMP-9/IgA and TIMP-1/IgA in renal tissues from 56 patients with IgA nephropathy（Lee levels I-V） was detected with immunofluorescence double-staining.Results The TIMP-1/IgA was lowly expressed while the MMP-9/IgA was highly expressed in glomeruli of patients with IgA nephropathy（Lee levels I-II）.The expression level of TIMP-1/IgA was higher while that of MMP-9/IgA was lower in glomeruli of patients with IgA neprhropathy（Lee level III）.The expression level of TIMP-1/IgA was the highest while that of MMP-9/IgA was lower in glomeruli of patients with IgA nephropathy（Lee level IV-V） and the lowest in sclerotic glomeruli of patients with IgA nephropathy.Conclusion The IgA deposition and TIMP-1 expression are increased while the MMP-9 expression is decreased during the progress of IgA nephropathy,which suggests that IgA deposition-induced imbalance of MMP-9/TIMP-1 is involved in glomerular sclerosis in patients with IgA nephropathy.

关 键 词：IGA肾病 基质金属蛋白酶-9 基质金属蛋白酶组织抑制因子-1 石蜡切片 免疫荧光双标记 

分 类 号：R612.31[医药卫生—外科学]