低温冷冻对皮肤高分子量角蛋白表达的影响  

Effect of cryopreservation on expression of high molecular weight epidermal keratin

作　　者：蔡宏[1] 董宁[2] 姚咏明[2] 李绍然[2] 贾晓明[2] 

机构地区：[1]空军总医院皮肤科,北京100036 [2]解放军总医院第一附属医院,北京100037

出　　处：《军医进修学院学报》2011年第12期1271-1273,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的研究皮肤低温储存后表皮层高分子量角蛋白的变化,从而分析皮肤组织细胞骨架的低温生物学效应。方法将新鲜成人皮肤分为新鲜组、4℃组、-20℃组、-80℃组和-196℃组,于冻存后第14天复温,运用免疫组织化学染色和电镜观察角蛋白的改变,并用图像分析仪测定表皮组织中的含量变化。结果随着保存温度的降低,4℃、-20℃、-80℃组的角蛋白均表达下降,而-196℃组与新鲜皮肤组表达相似。结论皮肤组织的低温损伤与细胞骨架结构密切相关,角蛋白在该低温生物学机制中发挥着重要作用。Objective To study the changes in cryo-preserved high molecular weight epidermal keratin and analyze the effect of cryobiology on epidermal cytoskeleton.Methods Adult human skin samples were divided into fresh group,4℃ group,-20℃ group,-80℃ group and-196℃ group.Changes in epidermal keratin were observed under an electron microscope with immunohistochemical staining 14 days after cryopreservation.Keratin level in skin tissue was analyzed with an image analyzer.Results The expression level of keratin decreased with the decreasing preservation temperature in 4℃ group,-20℃ group,and-80℃ group,and was similar in-196℃ group and fresh group.Conclusion Hypothermia injury of skin is closely correlated with cytoskeleton,and keratin plays an important role in the mechanism of cryobiology.

关 键 词：皮肤 高分子量角蛋白 低温 

分 类 号：R35[医药卫生—基础医学]