中药夏枯草对人甲状腺癌细胞系SW579增殖周期及凋亡的影响  

Effects of Traditional Chinese Medicine Prunella Vulgaris on Proliferation Cycle and Apoptosis of Human Thyroid Cancer Cell Line SW579

作　　者：张静[1] 王瑛 赵华栋[3] 张婧[1] 董旭才[1] 王超[1] 高军[1] 张惠中[1] 

机构地区：[1]第四军医大学唐都医院临床实验与检验输血科 [2]解放军61081部队门诊部 [3]第四军医大学唐都医院普通外科

出　　处：《现代生物医学进展》2011年第23期4434-4436,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30801121)

摘　　要：目的:探讨夏枯草对人甲状腺癌细胞系SW579细胞生长的抑制作用及其对细胞增殖周期和凋亡的影响。方法:采用甲基噻唑(MTT)比色法和生长曲线测定不同浓度夏枯草在不同作用时间内对在体外培养SW579细胞增殖的影响,同时应用流式细胞术检测细胞增殖周期及凋亡率的变化。结果:夏枯草可在G0/G1期阻滞人甲状腺癌细胞系SW579的增殖,使S期细胞比率降低;在一定范围内,夏枯草的浓度越高、作用时间越长,对肿瘤细胞生长的抑制作用越强,凋亡率也越高。结论:夏枯草能抑制人甲状腺癌细胞系SW579细胞生长,并诱导细胞凋亡而阻止细胞周期。Objective： To investigate the suppressive effect of Prunella vulgaris on the growth and the proliferation cycle and apoptosis of human thyroid cancer cell line SW57. Methods： The survival rate and the inhibitory rate of SW579 cell in vitro were detected by MTT colorimetrie assay and cell growth curve assay at different time points under different concentration of Prunella vulgaris; The cell proliferation cycle and the apoptotic rate were examined by flow cytometryanalysis. Results： Prunella vulgaris inhibited the proliferation of SW579 cell at G0/G1 phase, decreased the cell ratio at S phase. It suppressed the growth of tumor cells and raised the apoptotic rate in a concentration and time depending manner in a certain extent. Conclusion： Prunella vulgaris could suppress the proliferation of SW579 cell to induce apoptosis and block cell cycle.

关 键 词：夏枯草 甲状腺癌细胞系SW579 凋亡 

分 类 号：R285[医药卫生—中药学] R736.1[医药卫生—中医学]