RNA干扰对乙肝病毒基因的抑制作用  

RNA Interference Inhibitis Hepatitis B Virus Gene

作　　者：吕玉凤[1] 李晓光[1] 王晓燕[1] 

机构地区：[1]哈尔滨医科大学附属第二医院感染内科,黑龙江哈尔滨150008

出　　处：《现代生物医学进展》2011年第23期4569-4572,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30901258)

摘　　要：双链介导的遗传干涉的机制是1998年发现的。它通过双链RNA的介导特异性地降解相应序列从而导致转录后水平的基因沉默。RNA干扰作为后基因组时代的一种下调基因表达的工具已被广泛用于基因功能的研究以及疾病的治疗。利用小干扰RNA与乙肝病毒DNA通过共转染于HepG2肝癌细胞中使乙肝病毒基因沉默以达到抑制乙肝病毒复制作用。The mechanism of genetic interference directed by double-stranded RNA was first discovered in 1998. It degrades mRNA specifically and potently by the mediation of corresponding double-stranded RNA which leads to post-transcriptional gene silenc- ing. The use of the RNA interference （RNAi） to eliminate specific gene products has greatly facilitated the understanding of gene func- tion and the therapy of diseases. The use of the siRNA and hepaticas B virus-DNA （HBV-DNA）to cotransfected the liver cancer cell HepG2 leads to the HBV-DNA gene silencing inhibit HBV replication in cell culture.

关 键 词：乙型肝炎病毒 RNA干扰 基因沉默 转染 尾静脉注射 

分 类 号：R512.62[医药卫生—内科学]