小头飞虱属2个近缘种的ITS分析  

ITS Analysis of Malaxella(Hemiptera:Delphacidae) Based on Internal Transcribed Spacer-1

作　　者：侯晓晖[1,2] 陈祥盛[1] 

机构地区：[1]贵州大学昆虫研究所/贵州山地农业病虫害重点实验室,贵州贵阳550025 [2]遵义医学院,贵州遵义563003

出　　处：《贵州农业科学》2011年第11期104-106,109,共4页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"中国飞虱科系统发育及生物地理学研究"(30100015);"西南地区害竹头喙亚目昆虫物种多样性研究"(30560020);"中国竹子飞虱区系分类;DNA条形码及多媒体鉴定系统研究"(31160163);贵州省优秀青年科技人才基金项目"中国飞虱科物种多样性研究"[黔科合人字(2005)0520];贵州省优秀科技教育人才省长资金"贵州害竹叶蝉和飞虱种类详查"[黔省专合字(2005)357];贵州省国际科技合作项目"中加葡萄植原体病害媒介昆虫的鉴定与筛查"[黔科合外G字(2010)7005]

摘　　要：为了更准确地鉴别飞虱科近缘种类,采用DNA测序的方法获得小头飞虱属2个近缘种类的ITS1部分序列,并对其进行了分子鉴定。结果表明,黄小头飞虱和四刺小头飞虱ITS1序列平均长度分别为802bp和1 044bp,可初步区分二者;二者遗传距离在种内为0.005～0.035,种间为2.725～2.833,差异显著;系统发育重建显示2个近缘种类各自聚为1支,二者互为姐妹群。研究结果初步证实,通过对ITS1序列信息、遗传距离和系统发育分析等研究,可以准确鉴定飞虱科的近缘种类,进而解决形态学分类时而无法准确鉴定等问题。Molecular identification of two related species of bamboo-feeding planthopper genus Malaxella,M.flava and M.tetracantha,was studied based on internal transcribed spacer-1 to identify the related species of Delphacidae.The results showed the sequences length of M.flava and M.tetracantha were 802 and 1 044 respectively,genetic distance of interspecies and intraspecies were obviously different,both category were sister group in phylogenetic analysis.So accuracy identification was possible through the research of sequences information,genetic distance and phylogeny.Then these methods could be used for resolving the difficulty problems from morphological identification.

关 键 词：飞虱 近缘种 小头飞虱属 分子鉴定 ITS1 

分 类 号：S435.112[农业科学—农业昆虫与害虫防治]