免疫浊度法检测血清β_2微球蛋白在罗氏全自动生化分析仪上的应用评价  

Application Evaluation of Immunoturbidimetric Method for β_2-microglobulin on Roche Automatic Biochemical Analyzer

作　　者：席金瓯[1] 

机构地区：[1]湖北省妇幼保健院检验科,武汉430070

出　　处：《医疗卫生装备》2011年第11期84-85,共2页Chinese Medical Equipment Journal

摘　　要：目的:对免疫浊度法测定血清β2微球蛋白(β2-MG)进行性能评价,以确定其分析性能是否符合实验室应用的要求。方法:在罗氏全自动生化分析仪上对免疫浊度法测定血清β2微球蛋白(β2-MG)进行了灵敏度评价、精密度评价及抗干扰实验。结果:免疫浊度法测定β2-MG批内平均变异系数(CV)为1.74%,批间平均CV为2.79%,平均回收率为99.0%。血红蛋白(Hb)在2 g/L以下对测定结果无干扰。三酰甘油在(TG)4.5 mmol/L以下对测定结果无干扰。与日立7170全自动生化仪的免疫比浊法比较有良好的相关性,Y=0.904X+0.18,γ=0.923(P<0.01)。结论:免疫浊度法测定血清β2-MG具有较高的灵敏度和精密度,适于大批量及临床常规使用。Objective To evaluate the analysis capability of β2-microglobulin by Roche automatic biochemical analyzer with immunoturbidimetric method.Methods The sensitivity,precision and jamming test were used to evaluate immunoturbidimetric method for β2-microglobulin on Roche automatic biochemical analyzer.Results The mean CV for within-run assay was 1.74%,CV for between-run assay was 2.79%,and the average recovery was 99%.Low concentration of Hb（〈2 g/L） and TG（〈 4.5 mmol/L） didn＇t interfere with the detection results.Immunoturbidimetric method on Roche and Hitachi 7170 automatic biochemical analyzer showed good concordance in detection of β2-microglobulin（Y=0.904X＋0.18,γ=0.923,P〈0.01）.Conclusion Immunoturbidimetric method has a high sensitivity and precision which is suitable for automatic biochemical analyzer in routine laboratory.

关 键 词：免疫浊度法 Β2微球蛋白 性能评价 

分 类 号：R446.6[医药卫生—诊断学] R552[医药卫生—临床医学]