人重组白细胞介素-6可减少缺氧-复氧后大鼠海马培养神经元的DNA损伤  

rhIL-6 CAN DECREASE DNA DAMAGE OF CULTURED RAT HIPPOCAMPAL NEURONS AFTER ANOXIA AND REOXYGENATION

作　　者：丁爱石[1] 王福庄[1] 邹民吉[1] 范明[1] 

机构地区：[1]军事医学科学院基础医学研究所,神经生物学研究室北京100850

出　　处：《中国神经科学杂志》1999年第4期312-315,共4页

基　　金：国家自然科学基金!重点资助课题(39730190)

摘　　要：用原位末端标记（ＴＵＮＥＬ）法观察人重组白细胞介素－６（ｒｈＩＬ－６）对缺氧－复氧后大鼠海马培养神经元的ＤＮＡ损伤的影响。结果显示，海马神经元缺氧－复氧后ＤＮＡ损伤神经元百分率明显增高，经ｒｈＩＬ－６ 预处理的海马神经元缺氧－复氧后ＤＮＡ损伤神经元百分率明显低于对照组。本结果表明，缺氧－复氧能使体外培养海马神经元发生ＤＮＡ损伤，ｒｈＩＬ－６ 可减少缺氧－复氧后海马神经元的ＤＮＡ损伤。提示ｒｈＩＬ－６ 对海马神经元缺氧－复氧损伤可能具有一定保护作用。The effects of rhIL 6 on DNA damage were studied in cultured hippocampal neurons after anoxia reoxygenation by using the terminaldeoxy nucleotidyl transferase mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) method.The results showed that the percentage of the DNA damage neurons in rhIL 6 group was significantly less than those in control.The results indicated that rhIL 6 can decrease DNA damage of hippocampal neurons after anoxia reoxygenation.It is suggested that rhIL 6 may play a role in protecting neurons from damage during anoxia reoxygenation.

关 键 词：RHIL-6 海马神经元 缺氧 DNA损伤 大鼠 

分 类 号：R594.3[医药卫生—内科学] R361[医药卫生—临床医学]