脐血浆对正常骨髓造血刺激活性的研究  

Activities Assay of Umbilical Cord Blood Plasma on Hemopoietic Progenitors from Human Bone Marrow

作　　者：魏彩霞[1] 李玛琳[2] 汤正华[3] 肖敏[1] 贺金文[3] 杨绵本[1] 

机构地区：[1]昆明医学院第一附属医院血液科,昆明650032 [2]昆明医学院云南省天然药物药理重点实验室,昆明650031 [3]昆明医学院第一附属医院妇产科,昆明650032

出　　处：《昆明医学院学报》1999年第4期27-29,共3页Journal of Kunming Medical College

基　　金：国家自然科学基金;卫生部青年科学基金;云南省应用基础研究基金

摘　　要：为探讨脐血浆中造血活性物质的作用,用半固体培养法对混合脐血浆、重组人造血生长因子及正常成人混合血清在体外对正常骨髓造血祖细胞集落形成的情况进行了比较.结果显示,成人混合血清对粒单祖细胞及红系祖细胞的集落形成无明显的刺激活性,而混合脐血浆对粒单祖细胞及红系祖细胞的集落形成具有较高水平的刺激活性,其作用与小牛血清加人重组GM-CSF相当(P>005,差异无显著性).To investigate the hemopoietic effect of umbilical-cord blood plasma, the colony formation abilities of human bone marrow hemopoietic progenitors by mixed cord blood plasma, recombinant human hemopoietic growth factors and mixed adult serum were compared using semi-solid culture. The results showed that mixed adult serum has so significant activities on the colony formation of granular/mononcyte and erythroid lineage progenitors. In contrast, mixed cord blood plasma has relatively high level of activities on the colony formation of granular/mononcyte and erythroid lineage progenitors, and this effect is equal to that of newborn calf serum plus GM-CSF( P >0 05). Theses results suggested that umbilical-cord blood plasma has a high level of factors which could enhance hemopoiesis.

关 键 词：脐血 血浆 造血因子 骨髓 造血细胞 

分 类 号：R457[医药卫生—治疗学] Q462[医药卫生—临床医学]