怀山药茎尖脱毒培养与茎段增殖研究  

The Establishment of the System for Detoxified Huai Yam by Stem Tip Culture and Stem Cut Rapid Propagation

作　　者：苗利娟[1] 韩锁义[1] 张新友[1] 黄冰艳[1] 汤丰收[1] 董文召[1] 王素霞 

机构地区：[1]河南省农业科学院经济作物研究所,河南郑州450002 [2]温县农业局,河南温县454850

出　　处：《河南农业科学》2011年第11期123-125,共3页Journal of Henan Agricultural Sciences

基　　金：国家公益性行业科研专项(200903022)

摘　　要：以怀山药的茎尖、茎段为外植体,研究不同激素配比对山药茎尖分化及茎段快繁的影响,筛选适合于怀山药生长的培养基。结果表明:MS+BA 1mg/L+KT 2mg/L+NAA 0.2mg/L是怀山药茎尖分化较理想的培养基,成苗率较高(30%);MS+BA 1mg/L+NAA 0.5mg/L是怀山药茎段快繁最佳培养基,不定芽诱导率较高,成苗率最高,达到109.5%。添加0.01%聚乙烯吡咯烷酮(PVP)后茎段褐化现象得到了很大的改善。The effects of different hormone combinations on yam shoot tip differentiation and propagation were studied using shoot tip and stem cut as explants,to select the medium suited for Huai yam culture.The result showed that MS＋BA 1 mg/L＋KT 2 mg/L＋NAA 0.2 mg/L was an ideal medium for Huai yam tip differentiation with its high rate of seedling production（30%）.MS＋BA 1 mg/L＋NAA 0.5 mg/L was the best medium for rapid propagation by Huai yam stem cuts with its relatively high induction rate of adventitious buds and highest rate of seedling production（109.5%）.Addition of 0.01% PVP inhibited browning remarkably.

关 键 词：怀山药 茎尖 茎段 培养基 分化 脱毒 快繁 

分 类 号：S632.1[农业科学—蔬菜学]