血清B7-H3在脓毒血症患者体内的表达及其生物学意义  

Significant Elevation of Circulating B7-H3 in Sera from Patients with Sepsis and Its Biofunctions

作　　者：范德环[1] 张光波[2] 

机构地区：[1]河北医科大学附属唐山工人医院感染科,河北唐山063000 [2]苏州大学附属第一医院临床免疫学研究所,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2011年第5期769-771,795,共4页Suzhou University Journal of Medical Science

基　　金：江苏省卫生厅科研基金资助项目(H200961)

摘　　要：目的探讨可溶性B7-H3(sB7-H3)在脓毒血症患者体内的表达特征及其生物学意义。方法通过ELISA方法检测脓毒血症患者体内sB7-H3及其他炎症因子的表达水平,并对相关性进行分析。利用分离纯化的单核细胞,分析肿瘤坏死因子-α(TNF-α)在sB7-H3产生中的调控作用。利用B7-H3Ig融合蛋白,体外实验分析sB7-H3对单核细胞的生物学作用。结果脓毒血症患者与健康对照组相比,血清sB7-H3水平显著升高(P<0.01)。sB7-H3表达水平与TNF-α及基质金属蛋白酶-9(MMP-9)成显著正相关(分别P<0.01和<0.05)。TNF-α调控sB7-H3的产生,同时sB7-H3又可作用于单核细胞,促进TNF-α分泌表达(均P<0.01)。结论脓毒血症患者体内存在高水平sB7-H3,其水平受TNF-α调控,功能则表现为刺激单核细胞分泌TNF-α。Objective To measure levels of sB7-H3 in sera from septic patients with bacterial bloodstream infections and analyze its biofunctions.Methods Serum levels of sB7-H3,TNF-α,MMP-9,IL-17 and IFN-γ in septic patients were detected by ELISA.TNF-α were used to coculture with monocytes in vitro to analyze the regulation of sB7-H3 production.The effect of soluble B7-H3 on monocytes was also studied with B7-H3Ig fusion protein.Results A significant increased levels of sB7-H3 was found in patients with sepsis comapared with those in healthy subjects（P0.01）.The levels of circulating B7-H3 in septic patients correlated positively with TNF-α and MMP-9（P0.01 and0.05）,but not with IFN-γ or IL-17.The release of soluble B7-H3 was regulated by TNF-α.In turn,B7-H3Ig fusion protein was able to enhance TNF-α secretion from monocytes（all P0.01）.Conclusion The elevated circulating B7-H3 was found in patients with sepsis and might play an important role in inflammatory responses.

关 键 词：可溶性B7-H3 脓毒血症 单核细胞 TNF-Α 

分 类 号：R631.2[医药卫生—外科学]