丹参炮制前后特征图谱比较研究  

The Comparision of Fingerprint between Salviae Miltiorrhizae Radix et Rhizoma and its Prepared Samples

作　　者：陆丽[1] 

机构地区：[1]常熟市第二人民医院,江苏常熟215500

出　　处：《现代中药研究与实践》2011年第6期37-40,共4页Research and Practice on Chinese Medicines

摘　　要：目的采用HPLC法研究山东产丹参炮制前后指纹图谱变化,为科学评价及有效控制其质量提供可靠依据。方法以80%甲醇为提取溶剂,采用超声方法提取制备供试样品。以Hanbon C18色谱柱为固定相,乙腈:0.1%甲酸为流动相进行梯度洗脱,检测波长为280 nm,进样量20μL,流速为1.0 mL/min,柱温为25℃,进行HPLC指纹图谱检测,分析比较丹参炮制前后的指纹图谱。结果丹参经炮制后,指纹图谱中主要色谱峰发生了显著改变,与炮制前相比峰面积显著降低。表明丹参经炮制处理后,主要化学成分含量明显减少,炮制品共确定了12个共有峰。结论本研究结果建立了一种重现性、精密性良好的HPLC特征图谱检测方法,可用于丹参及其炮制品的质量控制。Objective To establish a sensitive and specific HPLC method for quality control of Salviae Miltiorrhiza Radix et Rhizoma.Methods HPLC was applied for quality assessment of Salviae Miltiorrhiza Radix et Rhizoma.HPLC analysis was performed under the following conditions： Hanbon C18 column（250 mm×4.6 mm,5 μm）,binary gradient mobile phase（10%～90% acetonitrile and 0.1% formic）,flow rate（1.0mL min-1）,injection volume（20 μL）,column temperature（25℃）,detection（280 nm）.Results The HPLC chromatographic fingerprinting of Salviae Miltiorrhiza Radix et Rhizoma,showing 12 characteristic peaks,was established from prepared samples of Salviae Miltiorrhiza Radix et Rhizoma.Conclusion The established HPLC fingerprint method was accurate and reliable,which could be used for the quality control of Salviae Miltiorrhiza Radix et Rhizoma and its prepared Samples.

关 键 词：丹参 炮制品 HPLC 特征图谱 

分 类 号：R284.1[医药卫生—中药学]