引起产科新生儿腹泻暴发的轮状病毒株VP4 VP7编码基因与其毒力关系的探讨  

A PRELIMINARY STUDY ON RELATIONSHIP BETWEEN THE VIRAL VIRULENCE AND VIRAL GENES ENCODING VP4 VP7 PROTEINS OF P2 [6] G4 ROTAVIRUSE THAT CAUSED A NEONATAL DIARRHEA OUTBREAK

作　　者：肖玮[1,2] 钱渊[1,2] 张又 

机构地区：[1]首都儿科研究所北京市感染与免疫中心实验室 [2]北京基因诊断基础性研究实验室,北京100020

出　　处：《病毒学报》1999年第4期314-322,共9页Chinese Journal of Virology

基　　金：卫生部科研基金;北京市自然科学基金

摘　　要：克隆并测定了引起产科新生儿腹泻暴发的Ｐ２［６］ 、Ｇ４ 型轮状病毒（ＢＮ 株）ＶＰ４ 的ＶＰ８片段和ＶＰ７ 编码基因的核苷酸序列，并据此推导出其氨基酸序列。与相应标准株和地方株（ 包括有毒株和无毒株） 比较的结果表明，所测ＶＰ８ 序列与相同型别（Ｐ２［６］） 的标准株Ｍ３７（ 无毒株） 和ＳＴ３（ 无毒株） 、地方株Ｎ１６（ 无毒株） 和ＶＥ７１５６（ 有毒株） 之间的同源性为９２－８ ％ ～９８－６ ％ ，胰酶作用位点各毒株间相同；位于ａａ４９ 、ａａ５０ 、ａａ５２ 、ａａ５３ 、ａａ７８ 处的氨基酸在有毒株与无毒株间（ 包括ＢＮ株） 不同，但分别保守。ＶＰ７ 基因与同型（Ｇ４） 标准株ＳＴ３（Ａ 亚型／ 无毒株） 和ＶＡ７０（Ｂ 亚型／ 有毒株） 、意大利地方株ＰＶ５２４９（Ａ 亚型／ 有毒株）和北京地方株ＣＲ１１７（ 有毒株） 、同型猪有毒株Ｇｏｔｔ 之间的同源性为９１－４ ％ ～９７－８ ％ ，其中与Ａ亚型的同源性为９５－５ ％ ～９６－３ ％ ，而与Ｂ亚型的同源性为９１－４ ％ ，提示ＶＰ７ 为Ｇ４Ａ 亚型，位于ａａ３８ 、ａａ７８ 、ａａ１４５ 、ａａ２３８ 位点的氨基酸在有毒株与无毒株之间不同，但分别保守。分析了虽为Ｐ２［６］ 型却反常地引起新生儿?The genes encoding VP8 (specific fragment from VP4 gene) and VP7 of BN rotavirus strain (serotype and genotype P2 [6] G4), which caused a neonatal diarrhea outbreak in Beijing, were cloned and sequenced, and their deduced amino acid sequences were analyzed. Amino acid homology of VP8 of BN strain (P2 [6] ) to corresponding type P2 [6] reference strains M37 (asymptomatic)、ST3 (asymptomatic) and field strains N16 (asymptomatic)、VE7156(symptomatic) ranged from 92.8-98.6%, all of these P2 [6] rotavirus strains (asymptomatic and symptomatic) had identical sequences in the trypsin cleavage sites. Amino acid homology of VP7 (G4) to corresponding serotype G4 reference strains ST3 (G4A/asymptomatic)、VA70 (G4B/symptomatic) and field strains PV5249 (G4A/symptomatic)、CR117 (symptomatic) and Gottfried strain of Porcine (G4/symptomatic) ranged from 91.4-97.8%, in which the homology to G4 A subtype rotaviruses (95.5-96.3%) was higher than that to G4 B subtype rotaviruses (91.4%), suggesting VP7 of BN strain belongs to A subtype for G4 serotype. The P2 [6] rotavirus used to be believed to associate with asymptomatic infections, but BN strain rotavirus belonging to P2 [6] type caused an diarrhea outbreak in neonates. The variation of the genes encoding VP8、VP7 and the correlation between variation and viral virulence were discussed.

关 键 词：轮状病毒 毒力 基因型 血清型 新生儿 腹泻 

分 类 号：R722.132[医药卫生—儿科] R373.9[医药卫生—临床医学]