中国大麦黄花叶病毒分离物的分子变异  

MOLECULAR VARIATIONS OF CHINESE ISOLATES OF BARLEY YELLOW MOSAIC BYMOVIRUS

作　　者：施农农[1] 陈剑平[1] M.J.Adams 

机构地区：[1]浙江省农业科学院,浙江杭州310021 [2]英国洛桑试验站

出　　处：《病毒学报》1999年第4期339-347,共9页Chinese Journal of Virology

基　　金：欧共体ISC项目;国家自然科学基金;浙江省青年科技人材专项资金

摘　　要：１３ 个供试的中国和英国大麦黄花叶病毒（ＢａＹＭＶ） 分离物，经ＲＮＡ１ 和ＲＮＡ２ 全基因组不同区域ＤＮＡ片段单链构象多态性分析（ＳＳＣＰ） ，外壳蛋白基因和ＲＮＡ２ ７０ｋＤ基因５＇端７０５ 碱基序列分析，以及此７０５ 碱基ＤＮＡ 片段限制性内切酶图谱分析结果，它们的ＲＮＡ１ 和ＲＮＡ２ 彼此无一相同，其中ＲＮＡ２ 变异比ＲＮＡ１ 更大。由于变异十分复杂，且没有规律性，因而当前通用的分子生物学技术尚不能简单地用于ＢａＹＭＶ 致病性分化或株系区分和检测。DNA fragments amplified from the bipartite genome of thirteen Chinese and UK isolates of barley yellow mosaic bymovirus(BaYMV) by RT-PCR were analysed by single-strand conformation polymorphism(SSCP). Variations in the coat protein gene and the 5'-terminal part(c. 705bp) of the 70kD regions of RNA2 of these BaYMV isolates were also revealed by sequencing and restriction mapping. The results indicated that none of all thirteen isolates was identical each other at the molecular level. Differences in RNA2 were greater than those in RNA1. The variations were very complicated so that none of modern molecular techniques could simply correlate the variations at nucleotide level with pathogenicity or strain differentiation.

关 键 词：大麦黄花叶病毒 分子变异 序列分析 RT-PCR SSCP 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治] Q78[农业科学—植物保护]