用AFLP技术研究花生根瘤菌的遗传多样性  

THE USE OF AFLP TECHNIQUE FOR THE STUDY OF GENETIC DIVERSITY IN PEANUT BRADYRHIZOBIA

作　　者：张小平[1] 陈强[1] 李登煜[1] LindstrmK 

机构地区：[1]四川农业大学农学院微生物系,雅安625000 [2]芬兰赫尔辛基大学应用化学与微生物系,00014

出　　处：《微生物学报》1999年第6期483-488,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金;欧盟国际合作项目

摘　　要：　采用ＡＦＬＰ分子标记技术，对分离自中国、津巴布韦、以色列的１３３ 株慢生花生根瘤菌（ Ｂｒａｄｙｒｈｉｚｏｂｉｕｍ ｓｐ．ａｒａｃｈｉｓ）和１３ 个代表菌株（ Ｂｒａｄｙｒｈｉｚｏｂｉｕｍ ｊａｐｏｎｉｃｕｍ ． Ｂｒａｄｙｒｈｉｚｏｂｉｕｍ ｅｌｋａｎｉｉ）的ＤＮＡ 扩增长度多态性进行了分析，并根据各供试菌株的遗传相似性进行了数值聚类。结果表明慢生花生根瘤菌群体内存在很高的遗传多样性，每个菌株的ＡＦＬＰ 带谱均与其它菌株完全不同。ＡＦＬＰ技术简便、快速、重现性极高，能表现高信息量的ＤＮＡ 长度多态性。A total of 146 bradyrhizobial strains including 133 peanut bradyrhizobia isolated from different regions and 13 reference strains representing \% B. japonicum\% and \%B.elkanii\% were studied for their genetic diversity by use of AFLP marker technique. The genomic similarity was analysed by numerical Taxonomy based on the AFLP patterns. The results showed that the genetic diversity of peanut bradyrhizbia were very high. Each strain had a identical AFLP pattern. AFLP technique is simple, fast, highly reproducible, and is a useful tool for evaluating genetic diversity.

关 键 词：花生 根瘤菌 AFLP 遗传多样性 聚类分析 

分 类 号：Q939.114[生物学—微生物学] Q933