禽病原性大肠杆菌1型菌毛的分离与鉴定  

THE ISOLATION AND CHARACTERIZATION OF TYPE 1 PILI FROMm PATHOGENIC ESCHERICHIA COLI OF CHICKEN ORIGIN 

作　　者：高崧[1] 姜焱[1] 刘业兵[1] 张如宽[1] 刘秀梵[1] 

机构地区：[1]扬州大学畜牧兽医学院动物医学系,扬州225009

出　　处：《微生物学报》1999年第6期521-526,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金

摘　　要：以旋涡混合法使禽病原性大肠杆菌分离株５６６ 、１７９４ 和ＴＫ３ 菌毛脱落，经硫酸铵沉淀、透析后进行蔗糖密度梯度离心，收集密度为１１０ 至１１５ｇ／ｃｍ３ 的蛋白带，经ＳＤＳＰＡＧＥ测定，３ 株菌菌毛蛋白的分子量分别在１７５ 、１７０ 和１７０ｋＤ；提纯菌毛保留了甘露糖敏感性凝集豚鼠红细胞的能力，证明它们为１ 型菌毛；从１７９４ 株提取的１ 型菌毛免疫ＢＡＬＢ／Ｃ 小鼠产生的高免血清在Ｗｅｓｔｅｒｎ ｂｌｏｔ 中与３ 个菌株的相应菌毛蛋白均呈阳性反应。上述结果表明，受检的３ 株禽病原性大肠杆菌均表达了１ 型菌毛，其分子量在１７５ ～１７０ｋＤ 之间，３ 个菌株的１The pili from pathogenic Escherichia coli isolates 566,1794 and TK3 of chicken and turkey origin were purified.After mechanic detachment from the bacterial cells,the pili were concentrated by precipitation with ammonium sulfate,dialyzed,and solubilized in buffer containing deoxycholate.The fraction containing the pilus was pruified further by ultracentrifugation in a sucrose gradient.After ultracentrifugation,the pili at the density of 1.10to 1.15g·cm -3 (between 10%～20% of sucrose gradients) were collected,and the purified pili from strain 566,1794 and TK\-3 had an apparent molecular weight of 17 500,17 000 and 17 000 respectively,which retained their ability to bind the erythrocyte in a mannose inhibitable fashion.Hyperimmunesera raised in BALB/C mice against the purified pili from strain 1794 reacted positively with type 1 pili from both isolates 566 and TK3 by immuno blot.These results revealed that the three strains either Chinese or north american isolates expressed type 1 pili which had molecular weights from 17 000 to 17 500,and they have common antigenic epitopes.

关 键 词：大肠杆菌 1型菌毛 分离 鉴定 禽源 病原菌 

分 类 号：S858.305.1[农业科学—临床兽医学] S852.6[农业科学—兽医学]