氧化苦参碱下调免疫细胞TLR7 mRNA的表达  

Expression of TLR7 mRNA in Mouse Immunocytes Down-regulated by Oxymatrine

作　　者：高小琪[1] 彭丽娜[1] 李力[2] 邱亚峰[1] 繆剑华 徐永莉[2] 史子学[1] 邵东华[1] 魏建超[1] 马志永[1] 

机构地区：[1]中国农业科学院上海兽医研究所公共卫生研究室,上海200241 [2]广西壮族自治区药用植物园,南宁530023

出　　处：《生物技术通报》2011年第11期150-153,159,共5页Biotechnology Bulletin

基　　金：2008年广西自然科学基金重点项目(桂科自08320152)

摘　　要：为了探讨氧化苦参碱对免疫细胞Toll-like receptor 7(TLR7)mRNA表达的影响,使用CCK-8试剂盒检测氧化苦参碱对RAW264.7细胞的生长抑制作用。应用荧光定量PCR方法检测氧化苦参碱对RAW264.7细胞TLR7 mRNA及其下游因子TNF-αmRNA表达的影响;检测氧化苦参碱对小鼠脾脏淋巴细胞TLR7 mRNA及其下游因子MyD88、TRAF-6 mRNA表达的影响。结果显示,氧化苦参碱对RAW264.7细胞的半数抑制率为5.9 mg/mL。氧化苦参碱下调RAW264.7细胞TLR7、TNF-αmRNA表达;下调小鼠脾脏淋巴细胞TLR7、MyD88、TRAF-6 mRNA表达。由此得出,氧化苦参碱可下调免疫细胞TLR7mRNA的表达。It was to evaluate the effect of oxymatrine on the transcription of toll-like receptor 7（TLR7） in mouse immunocytes.Murine macrophages（RAW264.7） were treated with oxymatrine at different concentrations ranging from 0.63 mg/mL to 20 mg/mL.The expression of TLR7 and its downstream genes such as MyD88,TRAF-6 and TNF-α in the transcriptional level was determined by Real-time PCR analysis.Treatment with oxymatrine resulted in the down-regulation of TLR7,MyD88,TRAF-6,TNF-α in mRNA level in RAW264.7 cells and splenic B lymphocytes.A significant decrease in the expression of TLR7 mRNA was observed in RAW264.7 cells from 3 h post treatment.The expression of TLR7,MyD88,TRAF-6 and TNF-α in mRNA level was detected in a dose-dependent manner in splenic B lymphocytes.Therefore,it proved that oxymatrine,an anti-inflammatory and anti-apoptotic substance from a traditional chinese medicine,was found to be effective in down-regulating the mRNA expression of TLR7,MyD88,TRAF-6 and TNF-α.

关 键 词：氧化苦参碱 TLR7免疫细胞 荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]