HPLC测定三黄汤中4种成分含量  

Determination of Four Components in Sanhuang Decoction by HPLC

作　　者：徐璐扬[1] 李中东[1] 刘庆丰[1] 施孝金[1] 钟明康[1] 

机构地区：[1]复旦大学附属华山医院临床药学研究室,上海200040

出　　处：《中国现代应用药学》2011年第12期1135-1138,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 建立高效液相色谱法测定三黄汤中4种重要活性成分（绿原酸、盐酸药根碱、盐酸小檗碱和盐酸巴马汀）的含量。方法 采用Agilent C18色谱柱（4.6 mm×200 mm，5 μm），流动相为1%醋酸甲醇溶液-1%醋酸溶液梯度洗脱；检测波长：329 nm；流速：1.0 mL·min^-1。结果 绿原酸、盐酸药根碱、盐酸小檗碱和盐酸巴马汀的线性范围分别为0.5-4.0 μg·mL^-1（r=0.999 1），0.5-4.0 μg·mL^-1（r=0.999 7），0.5-12.0 μg·mL^-1（r=0.999 3），0.3-4.0 μg·mL^-1（r=0.999 9）。平均回收率分别为99.0%（RSD=1.13%），99.3%（RSD=1.20%），99.0%（RSD=0.27%），99.8%（RSD=0.88%）。日内、日间RSD均〈2%。结论 本方法准确、灵敏、重复性好，可同时测定三黄汤中4种重要化学成分的含量。OBJECTIVE To establish an HPLC method for simultaneous determination of four important active components in Sanhuang decoction. METHODS An Agilent C18 column （4.6 mm×200 mm, 5 μm） was used with the mobile phase of 1% acetic acid methanol solution-1% acetic acid by gradient dilution, at the flow rate of 1.0 mL·min^-1 and detection wavelength of 329 nm. RESULTS The linear calibration curves of chlorogenic acid, jatrorrhizine hydrochloride, berberine hydrochloride and palmatine hydrochloride were obtained in the ranges of 0.5-4.0 μg·mL^-1（r=0.999 1）, 0.5-4.0 μg·mL^-1 （r=0.999 7）, 0.5-12.0 μg·mL^-1（r=0.999 3）, 0.3-4.0 μg·mL^-1 （r=0.999 9）, respectively. Their average recoveries （n=3） for the assay were 99.0%（RSD=1.13%）,99.3%（RSD=1.20%）,99.0%（RSD=0.27%） and 99.8%（RSD=0.88%）, respectively. The intra- and inter-day RSDs were all less than 2.0%. CONCLUSION This method is accurate, sensitive and suitable for quality control of Sanhuang decoction.

关 键 词：三黄汤 高效液相色谱法 绿原酸 盐酸药根碱 盐酸小檗碱 盐酸巴马汀 

分 类 号：R917.101[医药卫生—药物分析学]